Summary of Study ST002551

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001643. The data can be accessed directly via it's Project DOI: 10.21228/M8B41R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002551
Study TitleMetabolomics dataset of CNTF induced axon regeneration in mice post optic nerve crush
Study SummaryAxons are processes or extensions of a neuron that help connect one neuron with the next. In the eye all retinal ganglion cells (RGCs) reside within the retina but their axons travel a very long distance traversing through the optic nerve they connect with other neurons in the lateral geniculate nucleus in the brain. Loss of axons results in blindness in glaucoma and traumatic optic neuropathies. Optic nerve crush (ONC) is mouse is an assay system that enable pharmacological induction of axon regeneration from existing RGCs. Lipids form the outer boundary of axons, their synthesis or alterations are associated with metabolite changes. Our motivation was to understand what metabolite changes occurred when ONC axons regenerated due to ciliary neurotrophic factor (CNTF) treatment. We found metabolite profile changes associated with regeneration after crush induced by CNTF. This metabolite dataset was collected from C57Bl/6 mice expressing either AAV2-CNTF to promote regeneration or AAV2-Green Lantern as a control. Animals were subjected to optic nerve crush injury and allowed to recover for either 7 days or 14 days. At the respective time points, animals were euthanized and optic nerves were collected. Nerves underwent two rounds of extraction using a Precellys 24 Touch Homogenizer and a two solvent system of 1:1 Methanol/Water and 8:1:1 Acetonitrile/Methanol/Acetone. Metabolites were analyzed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) using a Vanquish Horizon Binary HPLC coupled to a Q Exactive Orbitrap mass spectrometer. Metabolites were identified using Compound Discoverer 3.3 and quantified using isotopic internal metabolite standards.
Institute
University of Miami
DepartmentMcKnight - Ophthalmology
LaboratoryBhattacharya Lab
Last NameBhattacharya
First NameSanjoy
Address1638 NW 10th Avenue, Room 706-A, Miami, FL 33136
Emailsbhattacharya@med.miami.edu
Phone3054824103
Submit Date2023-04-05
Num Groups4
Total Subjects23
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-04-21
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M8B41R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA256177175516_2_GREENL_CTL_OD_7dpi_NEGControl
SA256178158618_2_CNTF_CTL_OD_14dpi_POSControl
SA256179180969_2_CNTF_CTL_OD_7dpi_NEGControl
SA256180159201_3_CNTF_CTL_OD_14dpi_POSControl
SA256181180969_2_CNTF_CTL_OD_7dpi_POSControl
SA256182159200_1_GREENL_CTL_OD_14dpi_POSControl
SA256183175516_2_GREENL_CTL_OD_14dpi_POSControl
SA256184158618_2_CNTF_CTL_OD_7dpi_NEGControl
SA256185180969_1_CNTF_CTL_OD_7dpi_NEGControl
SA256186159201_1_CNTF_CTL_OD_7dpi_NEGControl
SA256187159200_3_GREENL_CTL_OD_14dpi_POSControl
SA256188180969_3_CNTF_CTL_OD_7dpi_POSControl
SA256189180968_1_GREENL_CTL_OD_7dpi_NEGControl
SA256190180969_3_CNTF_CTL_OD_7dpi_NEGControl
SA256191159200_3_GREENL_CTL_OD_7dpi_NEGControl
SA256192159201_3_CNTF_CTL_OD_7dpi_NEGControl
SA256193159200_1_GREENL_CTL_OD_7dpi_NEGControl
SA256194180968_1_GREENL_CTL_OD_7dpi_POSControl
SA256195180968_2_GREENL_CTL_OD_7dpi_POSControl
SA256196159201_1_CNTF_CTL_OD_14dpi_POSControl
SA256197180968_2_GREENL_CTL_OD_7dpi_NEGControl
SA256198180968_3_GREENL_CTL_OD_7dpi_NEGControl
SA256199180969_1_CNTF_CTL_OD_7dpi_POSControl
SA256200180968_3_GREENL_CTL_OD_7dpi_POSControl
SA256201158618_1_CNTF_ONC_OS_7dpi_NEGCrush
SA256202175516_1_GREENL_ONC_OS_7dpi_NEGCrush
SA256203180968_2_GREENL_ONC_OS_7dpi_NEGCrush
SA256204180968_3_GREENL_ONC_OS_7dpi_NEGCrush
SA256205180968_1_GREENL_ONC_OS_7dpi_NEGCrush
SA256206159201_2_CNTF_ONC_OS_7dpi_NEGCrush
SA256207159200_2_GREENL_ONC_OS_7dpi_NEGCrush
SA256208159200_3_GREENL_ONC_OS_7dpi_NEGCrush
SA256209159200_2_GREENL_ONC_OS_14dpi_POSCrush
SA256210180968_2_GREENL_ONC_OS_7dpi_POSCrush
SA256211180968_3_GREENL_ONC_OS_7dpi_POSCrush
SA256212180968_1_GREENL_ONC_OS_7dpi_POSCrush
SA256213180969_1_CNTF_ONC_OS_7dpi_NEGCrush
SA256214180969_3_CNTF_ONC_OS_7dpi_NEGCrush
SA256215180969_2_CNTF_ONC_OS_7dpi_NEGCrush
SA256216180969_1_CNTF_ONC_OS_7dpi_POSCrush
SA256217180969_2_CNTF_ONC_OS_7dpi_POSCrush
SA256218159201_2_CNTF_ONC_OS_14dpi_POSCrush
SA256219159201_3_CNTF_ONC_OS_14dpi_POSCrush
SA256220158618_1_CNTF_ONC_OS_14dpi_POSCrush
SA256221159200_3_GREENL_ONC_OS_14dpi_POSCrush
SA256222180969_3_CNTF_ONC_OS_7dpi_POSCrush
SA256223175516_1_GREENL_ONC_OS_14dpi_POSCrush
SA256224159201_3_CNTF_ONC_OS_7dpi_NEGCrush
SA256225Pooled_QC_5_NEGN/A
SA256226Pooled_QC_MS2_4_NEGN/A
SA256227System_Suitability_Blank_2_NEGN/A
SA256228Pooled_QC_MS2_3_NEGN/A
SA256229Pooled_QC_MS2_2_NEGN/A
SA256230Extraction_Blank_2_NEGN/A
SA256231Pooled_QC_MS2_1_NEGN/A
SA256232Pooled_QC_4_NEGN/A
SA256233System_Suitability_Blank_NEGN/A
SA256234Pooled_QC_MS2_2_POSN/A
SA256235Pooled_QC3_POSN/A
SA256236Pooled_QC_4_POSN/A
SA256237Pooled_QC_MS2_1_POSN/A
SA256238Pooled_QC2_POSN/A
SA256239QC1_POSN/A
SA256240Extraction_Blank_1_POSN/A
SA256241Pooled_QC1_POSN/A
SA256242Pooled_QC_5_POSN/A
SA256243Extraction_Blank_2_POSN/A
SA256244Extraction_Blank_1_NEGN/A
SA256245Pooled_QC1_NEGN/A
SA256246Pooled_QC2_NEGN/A
SA256247QC1_NEGN/A
SA256248System_Suitability_Blank_POSN/A
SA256249Pooled_QC_MS2_3_POSN/A
SA256250Pooled_QC_MS2_4_POSN/A
SA256251System_Suitability_Blank_2_POSN/A
SA256252Pooled_QC3_NEGN/A
Showing results 1 to 76 of 76
  logo