Summary of Study ST002551
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001643. The data can be accessed directly via it's Project DOI: 10.21228/M8B41R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002551 |
| Study Title | Metabolomics dataset of CNTF induced axon regeneration in mice post optic nerve crush |
| Study Summary | Axons are processes or extensions of a neuron that help connect one neuron with the next. In the eye all retinal ganglion cells (RGCs) reside within the retina but their axons travel a very long distance traversing through the optic nerve they connect with other neurons in the lateral geniculate nucleus in the brain. Loss of axons results in blindness in glaucoma and traumatic optic neuropathies. Optic nerve crush (ONC) is mouse is an assay system that enable pharmacological induction of axon regeneration from existing RGCs. Lipids form the outer boundary of axons, their synthesis or alterations are associated with metabolite changes. Our motivation was to understand what metabolite changes occurred when ONC axons regenerated due to ciliary neurotrophic factor (CNTF) treatment. We found metabolite profile changes associated with regeneration after crush induced by CNTF. This metabolite dataset was collected from C57Bl/6 mice expressing either AAV2-CNTF to promote regeneration or AAV2-Green Lantern as a control. Animals were subjected to optic nerve crush injury and allowed to recover for either 7 days or 14 days. At the respective time points, animals were euthanized and optic nerves were collected. Nerves underwent two rounds of extraction using a Precellys 24 Touch Homogenizer and a two solvent system of 1:1 Methanol/Water and 8:1:1 Acetonitrile/Methanol/Acetone. Metabolites were analyzed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) using a Vanquish Horizon Binary HPLC coupled to a Q Exactive Orbitrap mass spectrometer. Metabolites were identified using Compound Discoverer 3.3 and quantified using isotopic internal metabolite standards. |
| Institute | University of Miami |
| Department | McKnight - Ophthalmology |
| Laboratory | Bhattacharya Lab |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | 1638 NW 10th Avenue, Room 706-A, Miami, FL 33136 |
| sbhattacharya@med.miami.edu | |
| Phone | 3054824103 |
| Submit Date | 2023-04-05 |
| Num Groups | 4 |
| Total Subjects | 23 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-21 |
| Release Version | 1 |
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Project:
| Project ID: | PR001643 |
| Project DOI: | doi: 10.21228/M8B41R |
| Project Title: | Metabolomics dataset of CNTF induced axon regeneration in mice post optic nerve crush |
| Project Summary: | Untargeted metabolomics of AAV_CNTF mice at 7 days and 14 days post-crush, and AAV_Green Lantern mice at 7 days and 14 days post-crush. Both genders included. Metabolites were extracted in serial extraction method: 1) 1:1 Methanol/H2O; 2) 8:1:1 Acetonitrile/Methanol/Acetone. Metabolites were separated using hydrophilic interaction liquid chromatography (HILIC) and run on a Q Exactive mass spectrometer in both positive and negative modes. |
| Institute: | University of Miami |
| Department: | McKnight - Ophthalmology |
| Laboratory: | Bhattacharya Lab |
| Last Name: | Bhattacharya |
| First Name: | Sanjoy |
| Address: | 1638 NW 10th Avenue, Room 706-A, Miami, FL 33136 |
| Email: | sbhattacharya@med.miami.edu |
| Phone: | 3054824103 |
