Summary of Study ST002920

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001814. The data can be accessed directly via it's Project DOI: 10.21228/M87D9M This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002920
Study TitlePossible PPARG-independent effects of DINCH and MINCH on central carbon metabolism
Study SummaryIn the third part of the project, we investigated the PPARG-independent effects of DINCH and MINCH on the central carbon metabolism of human SGBS cells. SGBS preadipocytes were treated for 12 days with 10 µM MINCH, 10 µM DINCH, or rosiglitazone in the presence of the PPARG inhibitor GW9662. Irreversible blocking of PPARG was achieved by incubating cells with 10 µM GW9662 1 hour before treatment and maintaining a regular treatment interval of 2 days. In conclusion, although the PPARG inhibitor GW9662 greatly reduced the effects of MINCH and rosiglitazone, slightly increased lipid accumulation along with increased lactate secretion and increased concentrations of pyruvate cycle metabolites were consistently induced by MINCH treatment even after PPARG inhibition. This suggests that MINCH exerts its effects on lipid accumulation and central carbon metabolism at least in part via a PPARG-independent mechanism.
Institute
Helmholtz Centre for Environmental Research
Last NameEngelmann
First NameBeatrice
AddressPermoserstr. 15
Emailbeatrice.engelmann@ufz.de
Phone00493412351099
Submit Date2023-10-08
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2023-11-01
Release Version1
Beatrice Engelmann Beatrice Engelmann
https://dx.doi.org/10.21228/M87D9M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA316858Ctrl_GW_1Control GW9662 treatment
SA316859Ctrl_GW_4Control GW9662 treatment
SA316860Ctrl_GW_3Control GW9662 treatment
SA316861Ctrl_GW_2Control GW9662 treatment
SA316862Ctrl_GW_SN2Control GW9662 treatment supernatant
SA316863Ctrl_GW_SN4Control GW9662 treatment supernatant
SA316864Ctrl_GW_SN3Control GW9662 treatment supernatant
SA316865Ctrl_GW_SN1Control GW9662 treatment supernatant
SA316866DINCH_GW_4DINCH 10µM + GW9662 treatment
SA316867DINCH_GW_3DINCH 10µM + GW9662 treatment
SA316868DINCH_GW_1DINCH 10µM + GW9662 treatment
SA316869DINCH_GW_2DINCH 10µM + GW9662 treatment
SA316870DINCH_GW_SN3DINCH 10µM + GW9662 treatment supernatant
SA316871DINCH_GW_SN4DINCH 10µM + GW9662 treatment supernatant
SA316872DINCH_GW_SN2DINCH 10µM + GW9662 treatment supernatant
SA316873DINCH_GW_SN1DINCH 10µM + GW9662 treatment supernatant
SA316874MINCH_GW_4MINCH 10µM + GW9662 treatment
SA316875MINCH_GW_1MINCH 10µM + GW9662 treatment
SA316876MINCH_GW_2MINCH 10µM + GW9662 treatment
SA316877MINCH_GW_3MINCH 10µM + GW9662 treatment
SA316878MINCH_GW_SN2MINCH 10µM + GW9662 treatment supernatant
SA316879MINCH_GW_SN3MINCH 10µM + GW9662 treatment supernatant
SA316880MINCH_GW_SN4MINCH 10µM + GW9662 treatment supernatant
SA316881MINCH_GW__SN1MINCH 10µM + GW9662 treatment supernatant
SA316882Rosi_GW_4Rosi (d0-d4) + GW9662 treatment
SA316883Rosi_GW_1Rosi (d0-d4) + GW9662 treatment
SA316884Rosi_GW_3Rosi (d0-d4) + GW9662 treatment
SA316885Rosi_GW_2Rosi (d0-d4) + GW9662 treatment
SA316886Rosi_GW_SN4Rosi (d0-d4) + GW9662 treatment supernatant
SA316887Rosi_GW_SN1Rosi (d0-d4) + GW9662 treatment supernatant
SA316888Rosi_GW_SN2Rosi (d0-d4) + GW9662 treatment supernatant
SA316889Rosi_GW_SN3Rosi (d0-d4) + GW9662 treatment supernatant
Showing results 1 to 32 of 32
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