Summary of Study ST000040
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000018. The data can be accessed directly via it's Project DOI: 10.21228/M8Z59Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000040 |
| Study Title | Heatshock response of C. elegans using IROA (I) |
| Institute | University of Florida |
| Department | Biochemistry & Molecular Biology |
| Laboratory | Edison |
| Last Name | Stupp |
| First Name | Gregory |
| stuppie@ufl.edu | |
| Submit Date | 2013-12-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 3.2 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2014-03-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000060 | AN000061 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Accela 1250 | Thermo Accela 1250 |
| Column | Thermo Scientific Gold aQ | Thermo Scientific Gold aQ |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
MS:
| MS ID: | MS000041 |
| Analysis ID: | AN000060 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Samples were analyzed using a mass range of m/z 70-800 in positive and negative ionization mode, externally calibrated, using a Thermo Scientific Q-Exactive Orbitrap mass spectrometer equipped with an Open Accela autosampler and an Accela 1250 pump (San Jose, CA). The Q-Exactive was equipped with a heated electrospray ionization (HESI) source, which operated at a spray temperature of 500 °C, a spray voltage of 3 kV, and sheath and auxiliary gas flow rates of 60 and 10 arbitrary units, respectively. Features were extracted from the ms data using proprietary MATLAB IROA scripts. Ratios for each feature (heatshock/control) were calculated for each replicate. Ratios were normalized by setting the median log-fold change for each sample to zero |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 500 °C |
| Ion Spray Voltage: | 3 kV |
| Mass Accuracy: | 1.5 ppm |
| Scan Range Moverz: | 70-1000 |
| MS ID: | MS000042 |
| Analysis ID: | AN000061 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Samples were analyzed using a mass range of m/z 70-800 in positive and negative ionization mode, externally calibrated, using a Thermo Scientific Q-Exactive Orbitrap mass spectrometer equipped with an Open Accela autosampler and an Accela 1250 pump (San Jose, CA). The Q-Exactive was equipped with a heated electrospray ionization (HESI) source, which operated at a spray temperature of 500 °C, a spray voltage of 3 kV, and sheath and auxiliary gas flow rates of 60 and 10 arbitrary units, respectively. Features were extracted from the ms data using proprietary MATLAB IROA scripts. Ratios for each feature (heatshock/control) were calculated for each replicate. Ratios were normalized by setting the median log-fold change for each sample to zero |
| Ion Mode: | NEGATIVE |
| Ion Source Temperature: | 500 °C |
| Ion Spray Voltage: | 3 kV |
| Mass Accuracy: | 1.5 ppm |
| Scan Range Moverz: | 70-1000 |
