Summary of Study ST000040
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000018. The data can be accessed directly via it's Project DOI: 10.21228/M8Z59Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000040 |
| Study Title | Heatshock response of C. elegans using IROA (I) |
| Institute | University of Florida |
| Department | Biochemistry & Molecular Biology |
| Laboratory | Edison |
| Last Name | Stupp |
| First Name | Gregory |
| stuppie@ufl.edu | |
| Submit Date | 2013-12-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 3.2 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2014-03-05 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000058 |
| Treatment Summary: | Heat Shock: Two successive synchronous generations of wild-type C. elegans (N2) were grown in S-complete buffer at 22C containing 5% 13C-labeled E. coli. Upon reaching the young adult stage, the population was washed from E. coli using standard procedures and was divided into four replicates. Each replicate was treated with a 30 min heat shock at 33 °C followed by incubation at 22C for 1.5 hours. Control: Two successive synchronous generations of wild-type C. elegans (N2) were grown in S-complete buffer at 22C containing 95% 13C-labeled E. coli. Upon reaching the young adult stage, the population was washed from E. coli using standard procedures. |
| Treatment: | Abiotic |
| Treatment Dose: | 30C | 22C |
| Treatment Doseduration: | 30C for 30 minutes; 1.5 hours at 22 C before harvest | 22C for 30 minutes; 1.5 hours at 22 C before harvest |
| Animal Acclimation Duration: | - |
