Summary of Study ST001207
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000811. The data can be accessed directly via it's Project DOI: 10.21228/M8VD62 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001207 |
| Study Title | Lipidomics in the serum of cold exposed mice treated with 12-LOX inhibitor LOXBlock-1 |
| Study Summary | We aimed to investigate whether the cold-induced release of 12-LOX products into the circulation were dependent on 12-LOX activation. We pre-treated C57BL6/J mice with the pharmacological inhibitor LOXBlock-1 or its vehicle (DMSO), and after 15 minutes we placed them under cold temperature (5C) for 4 hours. A control group was injected with DMSO and kept at room temperature for the same 4 hours. After this period of time we, collected the blood, and obtained the serum fraction that was immediately frozen and submitted for untargeted lipidomics. |
| Institute | Joslin Diabetes Center |
| Last Name | Leiria |
| First Name | Luiz |
| Address | One Joslin Place, Boston-MA, 02215 |
| luiz.leiria@joslin.harvard.edu | |
| Phone | 617-309-1967 |
| Submit Date | 2019-06-26 |
| Num Groups | 3 |
| Study Comments | Joslin Diabetes Center affiliate of Harvard Medical School |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-07-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002009 |
|---|---|
| Chromatography ID | CH001453 |
| MS ID | MS001862 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Ekspert MicroLC 200 system |
| Column | Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um) |
| MS Type | ESI |
| MS instrument type | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE |
| Units | Peak Area |
MS:
| MS ID: | MS001862 |
| Analysis ID: | AN002009 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Full-scan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX). C18SPE cartridges were purchased from Biotage. All solvents were of HPLC or LC-MS/MS grade and were acquired from Sigma-Aldrich, Fisher Scientific, or VWR International. |
| Ion Mode: | NEGATIVE |
