Summary of Study ST001523

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001005. The data can be accessed directly via it's Project DOI: 10.21228/M8SH6G This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001523
Study TitleThe effect of fasting and sirtuin overexpression on serum metabolome.
Study Typeuntargeted metabolomics
Study SummaryThe levels of the sirtuins SIRT1 and SIRT6 are regulated by nutrient availability in several mammalian tissues. In addition, overexpressing SIRT1 in mice improves healthspan, while SIRT6 overexpression increases both healthspan and lifespan. However, little is known about the impact of these sirtuins at the in vivo metabolomics level. Thus, we performed metabolomics on serum taken from 15 months old male mice overexpressing SIRT1, SIRT6 and SIRT1+SIRT6 as well as WT controls. Sera were obtained from mice at three nutritional time points - at fed state, after 4h morning fast and after 16h fast.
Bar Ilan University
DepartmentThe Mina & Everard Goodman Faculty of Life Sciences
LaboratoryLaboratory of Prof. Haim Cohen
Last NameCohen
First NameHaim
AddressBar Ilan University, Ramat Gan, Israel, 5290002
Submit Date2020-09-10
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2021-03-11
Release Version1
Haim Cohen Haim Cohen application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN002541
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus IV GC
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type QTOF
MS instrument name Leco Pegasus IV TOF
Units normalized peak height


MS ID:MS002359
Analysis ID:AN002541
Instrument Name:Leco Pegasus IV TOF
Instrument Type:QTOF
MS Type:EI
MS Comments:A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da.