Summary of Study ST001811
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001145. The data can be accessed directly via it's Project DOI: 10.21228/M8Q12H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001811 |
| Study Title | Evidence that class I glutamine amidotransferase, GAT1_2.1, acts as a glutaminase in roots of Arabidopsis thaliana |
| Study Type | Targeted Metabolite Quantification |
| Study Summary | In this study, we use a targeted metabolite quantification approach to demonstrate the difference in quantities of pathway intermediates between wild type Arabidopsis roots and gat1_2.1 mutants using glutamine as organic nitrogen treatment and KNO3 and Glu treatments as negative and positive controls, respectively. |
| Institute | Agriculture and Agri-Food Canada |
| Department | London Research and Development Centre |
| Laboratory | Frederic Marsolais |
| Last Name | Kambhampati |
| First Name | Shrikaar |
| Address | 1391 Sandford St, London, ON N5V 4T3, Canada |
| shrikaar.k@gmail.com | |
| Phone | 3144025550 |
| Submit Date | 2021-06-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-06-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002935 | AN002936 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | umol g FW-1 | umol g FW-1 |
MS:
| MS ID: | MS002726 |
| Analysis ID: | AN002935 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The following heated electrospray ionization (HESI) conditions were optimized for the analysis of amino and organic acids: spray voltage, 3.9 kV (ESI+), 3.5 kV (ESI-); capillary temperature, 250 °C; probe heater temperature, 450 °C; sheath gas, 30 arbitrary units; auxiliary gas, 8 arbitrary units; and S-Lens RF level, 60%. Injections of 5 μl were used with a flow rate of 0.3 mL min-1. Compounds were detected and monitored using targeted MS/MS, spectra were collected at 17,500 resolution, AGC target 1e6, maximum IT 65 ms, isolation window of 1 m/z, normalized collision energy of 30, intensity threshold of 1.6e5 and 10s dynamic exclusion. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Targeted_Metabolite_Analysis.pdf |
| MS ID: | MS002727 |
| Analysis ID: | AN002936 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The following heated electrospray ionization (HESI) conditions were optimized for the analysis of amino and organic acids: spray voltage, 3.9 kV (ESI+), 3.5 kV (ESI-); capillary temperature, 250 °C; probe heater temperature, 450 °C; sheath gas, 30 arbitrary units; auxiliary gas, 8 arbitrary units; and S-Lens RF level, 60%. Injections of 5 μl were used with a flow rate of 0.3 mL min-1. Compounds were detected and monitored using targeted MS/MS, spectra were collected at 17,500 resolution, AGC target 1e6, maximum IT 65 ms, isolation window of 1 m/z, normalized collision energy of 30, intensity threshold of 1.6e5 and 10s dynamic exclusion. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Targeted_Metabolite_Analysis |
