Summary of Study ST001811

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001145. The data can be accessed directly via it's Project DOI: 10.21228/M8Q12H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001811
Study Title	Evidence that class I glutamine amidotransferase, GAT1_2.1, acts as a glutaminase in roots of Arabidopsis thaliana
Study Type	Targeted Metabolite Quantification
Study Summary	In this study, we use a targeted metabolite quantification approach to demonstrate the difference in quantities of pathway intermediates between wild type Arabidopsis roots and gat1_2.1 mutants using glutamine as organic nitrogen treatment and KNO3 and Glu treatments as negative and positive controls, respectively.
Institute	Agriculture and Agri-Food Canada
Department	London Research and Development Centre
Laboratory	Frederic Marsolais
Last Name	Kambhampati
First Name	Shrikaar
Address	1391 Sandford St, London, ON N5V 4T3, Canada
Email	shrikaar.k@gmail.com
Phone	3144025550
Submit Date	2021-06-01
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2021-06-16
Release Version	1

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Treatment:

Treatment ID:	TR001901
Treatment Summary:	Wild-type Arabidopsis ecotype Columbia and gat1_2.1 T-DNA insertion lines were used for Gln and Glu treatments. Plants were grown on vertical plates at 22 °C under continuous light (ca. 70 μmol m-2 s-2), as previously described by Ivanov et al. (2012) on a defined nutrient medium containing a final concentration of 10 mM potassium phosphate (pH 6.5), 5 mM KNO3, 2 mM MgSO4, 1 mM CaCl2, 125 μg FeNaEDTA, micronutrients (50 mM H3BO3, 12 mM MnSO4, 1 mM ZnCl2, 1 mM CuSO4 and 0.2 mM Na2MoO4), 1% sucrose and 1% agar [28]. Ten-day old seedlings were transferred to plates containing the same medium without nitrogen as control or 10 mM Gln as sole N source. After 2 h, root tissue was harvested, frozen in liquid N2 and stored at -80 °C until total metabolite extractions was carried out. For growth in Gln and Glu, the same media and growth conditions were used with the exception of 5 mM KNO3 being substituted with either 2 mM Gln or 2 mM Glu and tissue was collected after 10 days.

Treatment ID:

TR001901

Treatment Summary:

Wild-type Arabidopsis ecotype Columbia and gat1_2.1 T-DNA insertion lines were used for Gln and Glu treatments. Plants were grown on vertical plates at 22 °C under continuous light (ca. 70 μmol m-2 s-2), as previously described by Ivanov et al. (2012) on a defined nutrient medium containing a final concentration of 10 mM potassium phosphate (pH 6.5), 5 mM KNO3, 2 mM MgSO4, 1 mM CaCl2, 125 μg FeNaEDTA, micronutrients (50 mM H3BO3, 12 mM MnSO4, 1 mM ZnCl2, 1 mM CuSO4 and 0.2 mM Na2MoO4), 1% sucrose and 1% agar [28]. Ten-day old seedlings were transferred to plates containing the same medium without nitrogen as control or 10 mM Gln as sole N source. After 2 h, root tissue was harvested, frozen in liquid N2 and stored at -80 °C until total metabolite extractions was carried out. For growth in Gln and Glu, the same media and growth conditions were used with the exception of 5 mM KNO3 being substituted with either 2 mM Gln or 2 mM Glu and tissue was collected after 10 days.