Summary of Study ST002073
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001315. The data can be accessed directly via it's Project DOI: 10.21228/M8R11F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002073 |
Study Title | Profiling of the human intestinal microbiome and bile acids under physiologic conditions using an ingestible sampling device |
Study Summary | 15 human subjects were sample using an ingestible sampling device to target specific regions of the small intestine by using different capsule types (capsule types 1 to 4). Stool was also analyzed. |
Institute | University of California, Davis |
Last Name | Folz |
First Name | Jake |
Address | 451 Health Sciences Drive |
jfolz@ucdavis.edu | |
Phone | (530) 752-8129 |
Submit Date | 2022-02-01 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-12-15 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003380 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo TSQ Vantage |
Ion Mode | NEGATIVE |
Units | ng/mL |
MS:
MS ID: | MS003147 |
Analysis ID: | AN003380 |
Instrument Name: | Thermo TSQ Vantage |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | MRM scans were imported to Skyline58 software. Skyline performed peak integration for all analytes with given mass transitions and retention time windows (Table S2). The chromatogram for each analyte was manually checked to confirm correct peak integration. Peak area was exported for all analytes. Bile acid chemical structures were removed if there was not a convincing chromatographic peak observed in ≥1 sample. The ratio of analyte to its closest eluting internal standard was calculated and used for quantification. A linear model was fitted to standard curve points for each bile acid (R2>0.995 for all bile acids) and the model was applied to all samples and blanks to calculate concentrations. The average concentration reported for method blanks was subtracted from sample concentrations. Since multiple dilutions were analyzed for each sample, the measurement closest to the center of the standard curve (750 ng/mL) was used. Zero values were imputed with a concentration value between 0.001 and 0.1 ng/mL. Concentrations were reported as ng/mL for intestinal sample liquid supernatant, and ng/g for wet stool. |
Ion Mode: | NEGATIVE |