Summary of Study ST002111
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001337. The data can be accessed directly via it's Project DOI: 10.21228/M8WM52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002111 |
| Study Title | Metabolomics dataset of optogenetic axon regenerative mouse model post optic nerve crush |
| Study Summary | This metabolite dataset was collected from bacterial channel rhodopsin expressing transgenic mouse models subjected to optic nerve crush (ONC) with subsequent stimulation with light (promoting regeneration) or non-stimulation (lacking axon regeneration). ONC retains retinal ganglion cells within the retina, while degenerating axons. In transgenic bacterial channel rhodopsin expressing cells, light stimulation promotes regeneration. Genetically matched wild-type uninjured optic nerves, or control transgenic mice, were also analyzed. Metabolites were carefully extracted from finely minced optic nerve tissue using a solvent system (initial separation using 1:1 Methanol and H2O and second extraction using 8:1:1 of Acetonitrile:Acetone:Methanol). Untargeted liquid chromatography-mass spectrometry (LC-MS/MS) profiling was performed using fractionation on a Vanquish Horizon Binary UHPLC. Subsequent analyses were performed on an inline coupled Q-Exactive Orbitrap instrument. Metabolites were identified using Compound DiscovererTM software. Statistical analysis was performed using MetaboAnalyst 5.0. |
| Institute | University of Miami |
| Department | Ophthalmology |
| Last Name | Bhattacharya |
| First Name | Sanjoy K. |
| Address | 1638 NW 10th Avenue, Room 706-A, Miami, FL 33136 |
| sbhattacharya@med.miami.edu | |
| Phone | (305) 482-4103 |
| Submit Date | 2022-03-08 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-04-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003454 | AN003455 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore C18+ (100 x 2.1mm,1.5um) | Thermo Accucore C18+ (100 x 2.1mm,1.5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | μg/mL | μg/mL |
MS:
| MS ID: | MS003217 |
| Analysis ID: | AN003454 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolites were identified using Compound Discoverer 3.3 software. Protein quantification was performed and resulting peak intensities were used to quantify metabolite levels. Corrected peak intensities were imported to MetaboAnalyst 5.0 for statistical analysis. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003218 |
| Analysis ID: | AN003455 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolites were identified using Compound Discoverer 3.3 software. Protein quantification was performed and resulting peak intensities were used to quantify metabolite levels. Corrected peak intensities were imported to MetaboAnalyst 5.0 for statistical analysis. |
| Ion Mode: | NEGATIVE |
