Summary of Study ST002455
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001583. The data can be accessed directly via it's Project DOI: 10.21228/M82X4B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002455 |
| Study Title | Organism-Wide Analysis of Sepsis Reveals Mechanisms of Systemic Inflammation |
| Study Type | lipidomics analysis |
| Study Summary | Phospholipase A2 group V (PLA2G5) is a secretory and Ca2+-dependent lipolytic enzyme and is inducible during several pathologic conditions. However, it has been unknown how PLA2G5 plays a role in sepsis. To study the role of PLA2G5 in sepsis, we performed lipidomics analysis of plasma and tissues from LPS-injected mice with or without PLA2G5 blockade. Here, we showed that PLA2G5 is involved in the production of fatty acids such as oleic acid and linoleic acid, lysophospholipids such as lysophosphatidic acid, lysophosphatidylcholine, lysophatidylethanolamine, and lysophosphatidylserine species, and metabolites derived from polyunsaturated fatty acids such as arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, and linoleic acids during sepsis. Thus, PLA2G5 regulates selective lipid pathways during sepsis. |
| Institute | University of Chicago |
| Department | Pritzker School of Molecular Engineering |
| Laboratory | Chevrier lab |
| Last Name | Takahama |
| First Name | Michihiro |
| Address | 900 E 57th St,, Chicago, Illinois, 60637, USA |
| mtakahama@uchicago.edu | |
| Phone | 7732302766 |
| Submit Date | 2023-01-23 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004005 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 |
| Column | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 4000 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | intensity |
MS:
| MS ID: | MS003753 |
| Analysis ID: | AN004005 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The samples were applied to a Kinetex C18 column (Kinetex C18, 2.1 x 150 mm, 1.7 µm particle; Phenomenex, Inc., Torrance, CA, USA) connected with ESI-MS/MS on a liquid chromatography (NexeraX2 system; Shimadzu Co., Kyoto, Japan) coupled with a 4000Q-TRAP quadrupole-linear ion trap hybrid mass spectrometer (AB Sciex, Framingham, MA, USA). For analyses of free fatty acids (FFAs), lysophospholipids (LPLs) and phospholipids, the samples were applied to the column and separated by a step gradient with mobile phase A (acetonitrile/methanol/water =1/1/1 (v/v/v) containing 5 mM phosphoric acid and 1 mM ammonium formate) and mobile phase B (2-propanol containing 5 µM phosphoric acid and 1 mM ammonium formate) at a flow rate of 0.2 mL/min at 50°C. For analyses of oxygenated fatty acid metabolites, the samples were applied to the column and separated using a step gradient including mobile phase C (water containing 0.1 % acetic acid) and mobile phase D (acetonitrile/methanol = 4/1 (v/v)) at a flow rate of 0.2 mL/ min at 45°C. Identification of phospholipids, LPLs, FFAs, and oxygenated PUFAs (polyunsaturated fatty acids) metabolites was conducted by multiple reaction monitoring (MRM) transition, and quantification was performed based on the peak area of the MRM transition and the calibration curve obtained with an authentic standard for each compound. |
| Ion Mode: | UNSPECIFIED |
