Summary of Study ST002455

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001583. The data can be accessed directly via it's Project DOI: 10.21228/M82X4B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002455
Study Title	Organism-Wide Analysis of Sepsis Reveals Mechanisms of Systemic Inflammation
Study Type	lipidomics analysis
Study Summary	Phospholipase A2 group V (PLA2G5) is a secretory and Ca2+-dependent lipolytic enzyme and is inducible during several pathologic conditions. However, it has been unknown how PLA2G5 plays a role in sepsis. To study the role of PLA2G5 in sepsis, we performed lipidomics analysis of plasma and tissues from LPS-injected mice with or without PLA2G5 blockade. Here, we showed that PLA2G5 is involved in the production of fatty acids such as oleic acid and linoleic acid, lysophospholipids such as lysophosphatidic acid, lysophosphatidylcholine, lysophatidylethanolamine, and lysophosphatidylserine species, and metabolites derived from polyunsaturated fatty acids such as arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, and linoleic acids during sepsis. Thus, PLA2G5 regulates selective lipid pathways during sepsis.
Institute	University of Chicago
Department	Pritzker School of Molecular Engineering
Laboratory	Chevrier lab
Last Name	Takahama
First Name	Michihiro
Address	900 E 57th St,, Chicago, Illinois, 60637, USA
Email	mtakahama@uchicago.edu
Phone	7732302766
Submit Date	2023-01-23
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2024-01-23
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002550
Sampleprep Summary:	Tissues were mechanically homogenized with the Precellys 24 homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France) in methanol containing internal standards (500 pmol/sample of d4-labeled EPA, d5-labeled PGE2, LPC with a 17:0 fatty acyl chain (LPC17:0), and PC with two 14:0 fatty acyl chains (PE14:0-14:0)) and then incubated overnight at -20°C. For extraction of phospholipids and lysophospholipids, one-tenth of tissue lysates were added to 10 volumes of 20 mM Tris-HCl (pH 7.4) and were extracted using the method of Bligh and Dyer. For extraction of oxygenated fatty acid metabolites, nine-tenths of the tissue lysates were added to water (final methanol concentration of 10% (v/v)), and the lipids were extracted using an Oasis HLB cartridge (Waters, Milford, MA, USA).

Sampleprep ID:

SP002550

Sampleprep Summary:

Tissues were mechanically homogenized with the Precellys 24 homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France) in methanol containing internal standards (500 pmol/sample of d4-labeled EPA, d5-labeled PGE2, LPC with a 17:0 fatty acyl chain (LPC17:0), and PC with two 14:0 fatty acyl chains (PE14:0-14:0)) and then incubated overnight at -20°C. For extraction of phospholipids and lysophospholipids, one-tenth of tissue lysates were added to 10 volumes of 20 mM Tris-HCl (pH 7.4) and were extracted using the method of Bligh and Dyer. For extraction of oxygenated fatty acid metabolites, nine-tenths of the tissue lysates were added to water (final methanol concentration of 10% (v/v)), and the lipids were extracted using an Oasis HLB cartridge (Waters, Milford, MA, USA).