Summary of Study ST000314
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000253. The data can be accessed directly via it's Project DOI: 10.21228/M8302K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000314 |
Study Title | NSAID treatment alters the metabolomics profile of liver, kidney, lung, and heart in an experimental mouse model of heat stroke |
Study Type | Metabolomics |
Study Summary | The objective of this study is to exploit broad spectrum metabolomic analysis to identify new biomarkers of multi-organ damage that will improve heat stroke (HS) diagnosis and treatment. The central hypothesis is that HS will lead to significant alterations in multi-organ metabolomics profiles that will serve as markers of HS severity, which will be shifted and intensified further by the acute use of NSAIDs. To test this hypothesis, we will be performing broad spectrum metabolomics to identify alternations in the metabolic signatures of key organs (heart, liver, kidney, and lung) in a highly validated rodent HS model leveraging implantable radiotelemetry. We will then compare these results with already completed histological gene/protein expression analysis to determine the best metabolic markers of HS induced organ damage. The results from this study will aid in the identification of preventative measures to reduce HS risk, as well as in developing therapeutics to treat multi-organ damage and facilitate recovery. The proposed study will provide the first metabolic assessment of HS severity and NSAID use, which will support future studies in HS patients to validate novel biomarkers that will improve clinical assessment of organ damage and recovery. |
Institute | University of North Carolina |
Department | Systems and Translational Sciences |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2015-12-31 |
Num Groups | 83 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2016-12-31 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
NMR ID: | NM000062 |
Analysis ID: | AN000500 |
Instrument Name: | Bruker |
Instrument Type: | FT-NMR |
NMR Experiment Type: | Other |
Field Frequency Lock: | Deuterium |
Standard Concentration: | 0.5 mM |
Spectrometer Frequency: | 700 MHz |
NMR Probe: | 5 mm ATMA Cryoprobe |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm, 4 inch |
Shimming Method: | Topshim |
Pulse Sequence: | noesypr1d |
Water Suppression: | yes |
Receiver Gain: | 4.5 |
Offset Frequency: | 3299.5 |
Chemical Shift Ref Cpd: | DSS |
Temperature: | 298.1 K |
Number Of Scans: | 128 |
Dummy Scans: | 4 |
Acquisition Time: | 3.893 |
Spectral Width: | 12.0227 ppm, 8.417 Hz |
Num Data Points Acquired: | 65536 |
Real Data Points: | 65536 |
Line Broadening: | 0.5 Hz |
Zero Filling: | yes |
Apodization: | Lorentzian |
Baseline Correction Method: | Polynomial |
Chemical Shift Ref Std: | DSS-D6 |
Binned Increment: | 0.04 |
Binned Data Excluded Range: | 4.755 - 4.855 (water); 7.20 - 7.35 (Imidazole) |