Summary of Study ST000314
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000253. The data can be accessed directly via it's Project DOI: 10.21228/M8302K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000314 |
| Study Title | NSAID treatment alters the metabolomics profile of liver, kidney, lung, and heart in an experimental mouse model of heat stroke |
| Study Type | Metabolomics |
| Study Summary | The objective of this study is to exploit broad spectrum metabolomic analysis to identify new biomarkers of multi-organ damage that will improve heat stroke (HS) diagnosis and treatment. The central hypothesis is that HS will lead to significant alterations in multi-organ metabolomics profiles that will serve as markers of HS severity, which will be shifted and intensified further by the acute use of NSAIDs. To test this hypothesis, we will be performing broad spectrum metabolomics to identify alternations in the metabolic signatures of key organs (heart, liver, kidney, and lung) in a highly validated rodent HS model leveraging implantable radiotelemetry. We will then compare these results with already completed histological gene/protein expression analysis to determine the best metabolic markers of HS induced organ damage. The results from this study will aid in the identification of preventative measures to reduce HS risk, as well as in developing therapeutics to treat multi-organ damage and facilitate recovery. The proposed study will provide the first metabolic assessment of HS severity and NSAID use, which will support future studies in HS patients to validate novel biomarkers that will improve clinical assessment of organ damage and recovery. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2015-12-31 |
| Num Groups | 83 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2016-12-31 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000348 |
| Treatment Summary: | Mouse heat stroke model accurately simulates the thermoregulatory, inflammatory, and organ damage responses observed in HS patients. Mice were Surgically implanted with radiotelemetry devices for the continual recording of core temperatrue during heat expsoure and 24 hours of HS recorvery. Mice were orally trated with vehicle and no drug (bacon-flavored treat) or indomethacin (5 mg/kg contained in a bacon-flavored treat) immediately prior to expsoure to a heated chamger (Model 3950, Therma Forma, Marietta, OH; ambient temperature; = 39.5 degrees celsius). Mice remained in the heated chamber, in the absence of food and water, until a maximum of 42.4 degrees celsius was reached. At the maximum temperature mice were removed from the heat and either sacrificed or provided ad libitum food and water until sacrifice at ~3 hours (when mice displayed maximum hypothermia depth; ~30 degrees C) or 24 hours of recovery (24 hrs after the start of heat exposure) when ~1.0 C fever was displayed. Heart, liver, lung, and kidney were rapidly excised, sliced into transverse or longitudinal sections, and fixed in 10% neutral-buffered formalin for histological analysis (Carson Millonig Formulation, Fisher Scientific, Springfield, MA) or stored at -80 degrees celcius for molecular and metabolomics analysis. |
| Treatment Protocol ID: | USARIEM Protocol A1002 - NSAIDS (INDO) |
| Treatment Compound: | Indomethacin |
| Treatment Route: | Oral |
| Treatment Dose: | 5 mg/kg |
| Treatment Vehicle: | Bacon-flavored treat |
| Animal Endp Tissue Coll List: | Heart, Liver, lungs and Kidneys |
