Summary of Study ST001476
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001000. The data can be accessed directly via it's Project DOI: 10.21228/M8F982 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001476 |
| Study Title | Design of Experiments for Maximizing T cell endpoints |
| Study Summary | Purified T cells from a single healthy donor were expanded in vitro with either magnetic beads or degradable micro-scaffold (DMS) particles. Magnetic bead cultures were expanded according to manufacturer’s instructions, while DMS cultures were expanded with varying DMS particle concentration, IL2 concentration, and antigen surface density on the particles, according to a design of experiments. Media of each culture of was sampled repeatedly over the course of a 14 day period. Quantities and characteristics of activated T cells were assessed at the end of the culture period, and media was analyzed by 1H-NMR. Analysis of spectra resulted in a set of 20 features that was used in predictive modeling of T cell endpoints, along with culture parameters described above and cytokine data. A second validation experiment was performed with different values for culture parameters, using the same culture, sampling, and NMR analysis methods. |
| Institute | University of Georgia |
| Department | Complex Carbohydrate Research Center |
| Laboratory | Edison |
| Last Name | Colonna |
| First Name | Max |
| Address | 315 Riverbend Rd |
| maxwellbaca@uga.edu | |
| Phone | 7065420257 |
| Submit Date | 2020-08-12 |
| Num Groups | 2 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Analysis Type Detail | NMR |
| Release Date | 2020-09-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000183 |
| Analysis ID: | AN002452 |
| Instrument Name: | Bruker Avance III HD |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | Deuterium |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 5mm TCI |
| NMR Solvent: | 90% H2O, 10% D2O |
| NMR Tube Size: | 1.7mm |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | Presaturation |
| Pulse Width: | 7.45 us |
| Power Level: | 5.5584 W |
| Receiver Gain: | 203 |
| Offset Frequency: | 2819.9 Hz |
| Presaturation Power Level: | 1.2333e-05 W |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 25 |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.72 s |
| Relaxation Delay: | 4 s |
| Spectral Width: | 20 ppm |
| Num Data Points Acquired: | 65382 |
| Line Broadening: | 0.3 Hz |
| Baseline Correction Method: | Polynomial order 5 |
