Summary of Study ST001476

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001000. The data can be accessed directly via it's Project DOI: 10.21228/M8F982 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001476
Study TitleDesign of Experiments for Maximizing T cell endpoints
Study SummaryPurified T cells from a single healthy donor were expanded in vitro with either magnetic beads or degradable micro-scaffold (DMS) particles. Magnetic bead cultures were expanded according to manufacturer’s instructions, while DMS cultures were expanded with varying DMS particle concentration, IL2 concentration, and antigen surface density on the particles, according to a design of experiments. Media of each culture of was sampled repeatedly over the course of a 14 day period. Quantities and characteristics of activated T cells were assessed at the end of the culture period, and media was analyzed by 1H-NMR. Analysis of spectra resulted in a set of 20 features that was used in predictive modeling of T cell endpoints, along with culture parameters described above and cytokine data. A second validation experiment was performed with different values for culture parameters, using the same culture, sampling, and NMR analysis methods.
Institute
University of Georgia
DepartmentComplex Carbohydrate Research Center
LaboratoryEdison
Last NameColonna
First NameMax
Address315 Riverbend Rd
Emailmaxwellbaca@uga.edu
Phone7065420257
Submit Date2020-08-12
Num Groups2
Total Subjects1
Raw Data AvailableYes
Analysis Type DetailNMR
Release Date2020-09-10
Release Version1
Max Colonna Max Colonna
https://dx.doi.org/10.21228/M8F982
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001565
Treatment Summary:Cultures were prepared with varied IL2 concentration, DMS concentration, and DMS surface densities as described in study design. Culture media was replenished on days 4, 6, 8, 11 after sampling.
Cell Media:Miltenyi Texmacs (Cat. 170-076-307)
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