Summary of Study ST002868
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001791. The data can be accessed directly via it's Project DOI: 10.21228/M86M7M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002868 |
| Study Title | Pathogenic Staphylococcus epidermidis ICE25 response to skin and blood pH |
| Study Summary | Staphylococcus epidermidis (SE) is one of the most common bacteria of the human skin microbiota. Despite its role as a commensal, SE has emerged as an opportunistic pathogen, associated with 80% of medical devices related infections. Moreover, these bacteria are extremely difficult to treat due to their ability to form biofilms and accumulate resistance to almost all classes of antimicrobials developed so far. Thus new preventive and therapeutic strategies are urgently needed. In spite of its clinical importance, the molecular mechanisms associated with SE colonisation and disease are still poorly understood. A deeper understanding of the metabolic and cellular processes associated with response to environmental factors characteristic of SE ecological niches in health and disease might provide new clues on colonisation and disease processes. Here we studied the impact of pH conditions, mimicking the skin pH (5.5) and blood pH (7.4), in a S. epidermidis pathogenic strain, belonging to the A/C clonal lineage, by means of next-generation proteomics and 1H NMR-based metabolomics. Moreover, we evaluated the metabolic changes occurring when a sudden pH change arise, simulating the skin barrier break produced by a catheter. |
| Institute | ITQB NOVA |
| Last Name | Gonçalves |
| First Name | Luís G. |
| Address | Avenida Republica |
| lgafeira@itqb.unl.pt | |
| Phone | 214469464 |
| Submit Date | 2023-09-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2024-01-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000267 |
| Analysis ID: | AN004701 |
| Instrument Name: | Bruker Avance II+ 800 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 800 |
| NMR Probe: | 5 mm TXI-Z H/C/N/-D |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | Topshim |
| Chemical Shift Ref Cpd: | TSP |
| Temperature: | 25 |
