Summary of Study ST001057

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000710. The data can be accessed directly via it's Project DOI: 10.21228/M8WT20 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001057
Study TitleMetabolite Extractions from Cyanobacteria
Study SummaryMetabolite Extractions data from the Strains Synechococcus sp. PCC 7002, Synechococcus elongatus PCC 11801 (New strain, Manuscript submitted) and Synechococcus elongatus PCC 7942
Indian Institute of Technology Bombay
DepartmentDepartment of Chemical Engineering
LaboratoryWangikar Laboratory
Last NameWangikar
First NamePramod
AddressRoom No. 136, Biosystems Lab
Submit Date2018-09-08
Publications An improved method for extraction of polar and charged metabolites from cyanobacteria.
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2018-09-27
Release Version1
Pramod Wangikar Pramod Wangikar application/zip

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Project ID:PR000710
Project DOI:doi: 10.21228/M8WT20
Project Title:Improved Extraction method of Polar and Charged Metabolites from Cyanobacteria.
Project Summary:We report excellent extraction method for cyanobacteria of the genus Synechococcus, viz., Synechococcus sp. PCC 7002, Synechococcus elongatus PCC 7942, and a newly isolated Synechococcus elongatus PCC 11801 (manuscript under review). High resolution-LC/MS was used to assess the relative abundance of the extracted metabolites. The different solvent systems used for extraction led to statistically significant changes in the extraction efficiency for a large number of metabolites. While a few hundred m/z features or potential metabolites were detected with different solvent systems, the abundance of over a quarter of all metabolites varied significantly from one solvent system to another. Further, the extraction methods were evaluated for a targeted set of metabolites that are important in 13C-MFA studies of photosynthetic organisms. While for the strain PCC 7002, the reported method using methanol-chloroform-water system gave satisfactory results, a mild base in the form of NH4OH had to be used in place of water to achieve adequate levels of extraction for PCC 7942 and PCC 11801.
Institute:Indian Institute of Technology Bombay
Department:Department of Chemical Engineering
Laboratory:Wangikar Laboratory
Last Name:Wangikar
First Name:Pramod
Address:Room No. 136, Biosystems Lab