Summary of Study ST001057

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000710. The data can be accessed directly via it's Project DOI: 10.21228/M8WT20 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001057
Study TitleMetabolite Extractions from Cyanobacteria
Study SummaryMetabolite Extractions data from the Strains Synechococcus sp. PCC 7002, Synechococcus elongatus PCC 11801 (New strain, Manuscript submitted) and Synechococcus elongatus PCC 7942
Indian Institute of Technology Bombay
DepartmentDepartment of Chemical Engineering
LaboratoryWangikar Laboratory
Last NameWangikar
First NamePramod
AddressRoom No. 136, Biosystems Lab
Submit Date2018-09-08
Publications An improved method for extraction of polar and charged metabolites from cyanobacteria.
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2018-09-27
Release Version1
Pramod Wangikar Pramod Wangikar application/zip

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Sample Preparation:

Sampleprep ID:SP001106
Sampleprep Summary:The cell suspension was filtered through a 0.8 µm nylon membrane and the filtered cells was quickly placed in 1.6 ml cold methanol (100%) or methanol-water mixture (80:20 v/v). The mixture was incubated at -80 °C for 1 hour following which the cell-solvent mixture was removed and placed in a 15 mL centrifuge tube. The cells sticking to the filter were then scraped off with chloroform (2.4 mL) and mixed with the cell-solvent mixture. The mixture was vortexed for 20 minutes under cold conditions (vortexed for 30s and kept on ice for 1 minute and the cycle repeated). Two mL of MilliQ water or NH4OH solution in water (at concentrations of 0.2 M, 0.4 M) was added into the mixture to separate the phases. The mixture was vortexed for 10 minutes, centrifuged for 15 minutes at 3200g at 4 °C to obtain two separate phases and the top aqueous-rich layer was collected (approximately 3 mL), evaporated to dryness and stored at -80 °C until further analysis. The stored pellets were reconstituted in 100 μL of 50/50 methanol-water mixture and filtered before injecting on LC/MS.