Summary of Study ST002053
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001297. The data can be accessed directly via it's Project DOI: 10.21228/M82H7B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002053 |
| Study Title | Resistance to NaFAc of in vitro maturated Toxoplasma gondii bradyzoites in human myotubes. |
| Study Summary | The apicomplexan parasite Toxoplasma gondii forms bradyzoite-containing tissue cysts that cause chronic and drug-tolerant infections. Here, we developed a human myotube-based in vitro culture model of functionally mature tissue cysts. In contrast to fast replicating tachyzoite forms of T. gondii these tissue cysts tolerate exposure to the aconitase inhibitor sodium fluoroacetate. These data characterize bradyzoites and tachyzoites treated with NaFAc. |
| Institute | Robert Koch-Institute |
| Last Name | Blume |
| First Name | Martin |
| Address | Seestr. 10, Berlin, Berlin, 13353, Germany |
| blumem@rki.de | |
| Phone | +49 30 18754 2572 |
| Submit Date | 2022-01-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-02-28 |
| Release Version | 1 |
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Project:
| Project ID: | PR001297 |
| Project DOI: | doi: 10.21228/M82H7B |
| Project Title: | In vitro maturation of Toxoplasma gondii bradyzoites in human myotubes and their metabolomic characterization |
| Project Type: | characterization of in vitro T. gondii stages |
| Project Summary: | The apicomplexan parasite Toxoplasma gondii forms bradyzoite-containing tissue cysts that cause chronic and drug-tolerant infections. Here, we developed a human myotube-based in vitro culture model of functionally mature tissue cysts. Metabolomic characterization of purified cysts reveals global changes that comprise increased levels of amino acids and decreased abundance of nucleobase- and tricarboxylic acid cycle-associated metabolites. In contrast to fast replicating tachyzoite forms of T. gondii these tissue cysts tolerate exposure to the aconitase inhibitor sodium fluoroacetate. |
| Institute: | Robert Koch-Institut |
| Last Name: | Blume |
| First Name: | Martin |
| Address: | Seestr. 10, Berlin, Berlin, 13353, Germany |
| Email: | blumem@rki.de |
| Phone: | +49 30 18754 2572 |
