Summary of Study ST002525
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001626. The data can be accessed directly via it's Project DOI: 10.21228/M8HT4B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002525 |
| Study Title | In situ microwave fixation provides an instantaneous snapshot of the brain metabolome - Part 2 |
| Study Summary | We demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection prior to preservation by liquid nitrogen that is not observed with microwave fixation. Next, microwave fixation was employed to define the impact of brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple regions of the mouse brain, evidenced by reduced 13C enrichment into glycogen, glycolysis, and the TCA cycle. Reduced glucose metabolism correlated with a marked decrease in GLUT2 expression and several metabolic enzymes in unique brain regions. In conclusion, our study supports the incorporation of microwave fixation to study terminal brain metabolism in rodent models. |
| Institute | University of Florida |
| Last Name | Sun |
| First Name | Ramon |
| Address | 1200 Newell Drive, ARB |
| ramonsun@ufl.edu | |
| Phone | 3522948407 |
| Submit Date | 2023-03-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-04-17 |
| Release Version | 1 |
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Project:
| Project ID: | PR001626 |
| Project DOI: | doi: 10.21228/M8HT4B |
| Project Title: | In situ microwave fixation provides an instantaneous snapshot of the brain metabolome |
| Project Summary: | The brain metabolome directly connects to brain physiology and neuronal function. Brain glucose metabolism is highly heterogeneous among brain regions and continues postmortem. Therefore, challenges remain to capture an accurate snapshot of the physiological brain metabolome in healthy and diseased rodent models. To overcome this barrier, we employed high-power focused microwave for the simultaneous euthanasia and fixation of mouse brain tissue to preserve metabolite pools prior to surgical removal and dissection of brain regions. We demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection prior to preservation by liquid nitrogen that is not observed with microwave fixation. Next, microwave fixation was employed to define the impact of brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple regions of the mouse brain, evidenced by reduced 13C enrichment into glycogen, glycolysis, and the TCA cycle. Reduced glucose metabolism correlated with a marked decrease in GLUT2 expression and several metabolic enzymes in unique brain regions. In conclusion, our study supports the incorporation of microwave fixation to study terminal brain metabolism in rodent models. |
| Institute: | University of Florida |
| Department: | Biochemistry and Molecular Biology |
| Laboratory: | Sun lab |
| Last Name: | Sun |
| First Name: | Ramon |
| Address: | 1200 Newell Drive, ARB |
| Email: | ramonsun@ufl.edu |
| Phone: | 3522948407 |
