Summary of Study ST002525
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001626. The data can be accessed directly via it's Project DOI: 10.21228/M8HT4B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002525 |
Study Title | In situ microwave fixation provides an instantaneous snapshot of the brain metabolome - Part 2 |
Study Summary | We demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection prior to preservation by liquid nitrogen that is not observed with microwave fixation. Next, microwave fixation was employed to define the impact of brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple regions of the mouse brain, evidenced by reduced 13C enrichment into glycogen, glycolysis, and the TCA cycle. Reduced glucose metabolism correlated with a marked decrease in GLUT2 expression and several metabolic enzymes in unique brain regions. In conclusion, our study supports the incorporation of microwave fixation to study terminal brain metabolism in rodent models. |
Institute | University of Florida |
Last Name | Sun |
First Name | Ramon |
Address | 1200 Newell Drive, ARB |
ramonsun@ufl.edu | |
Phone | 3522948407 |
Submit Date | 2023-03-22 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2023-04-17 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002631 |
Sampleprep Summary: | Brains were removed immediately post-mortem, and washed once with PBS, twice with diH2O, blotted dry, and snap frozen in liquid nitrogen. Other set of brains were snap frozen after microwave fixation as described above. The frozen tissues were pulverized to 10 μm particles in liquid N2 using a Freezer/Mill Cryogenic Grinder (SPEX SamplePrep). Brain regions were extracted with 1ml of 50% methanol in the grinder, while for muscle and liver twenty milligrams of each pulverized tissue were extracted in 1ml of 50% methanol and separated into polar (aqueous layer), and protein/DNA/RNA/glycogen pellet. The polar fraction was dried at 10-3 mBar using a SpeedVac (Thermo) followed by derivatization. |