Summary of Study ST000050
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000048. The data can be accessed directly via it's Project DOI: 10.21228/M8101K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000050 |
Study Title | Preterm Neonatal Urinary Renal Developmental and acute kidney injury Metabolomic Profiling |
Study Type | Metabolomics |
Study Summary | Brophy and Askenazi hypothesize that postnatal renal maturation results in specific identifiable patterns of urinary metabolites and that these profiles will be altered in the presence of AKI. Their long-term goal is to identify novel metabolomics urinary profiles that can provide real-time evidence of evolving neonatal renal injury thereby allowing earlier diagnosis and treatment.This study includes 40 pre-term infants age 2 days. Twenty infants were identified as not having AKI (11 females, mean gestational age=182.8 days, mean birth weight=834.0 grams) and 20 infants were identified as having AKI (13 females, mean gestational age=183.9 days, mean birth weight=815.8 grams). There are no statistical differences between the two groups based on gender, gestational age, and birth weight. |
Institute | University of North Carolina |
Department | RTI RCMRC |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2014-04-30 |
Num Groups | 2 |
Total Subjects | 40 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Uploaded File Size | 28 M |
Analysis Type Detail | NMR |
Release Date | 2018-08-27 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000064 |
Sampleprep Summary: | Frozen urine study samples were thawed on ice and vortexed for 30 seconds. Each of the 40 urine samples (200 µL) were prepared individually by addition of 25 µL D20 and 25 µL Chenomx Internal Standard (Chenomx ISTD, Edmonton, Alberta, Canada). Phenotypic pools for AKI and no AKI were generated with 60 µL of each urine sample pooled, mixed, divided into 3 aliquots. Additionally, 400 µL from both phenotypic pools were mixed to form a total pooled sample and divided into 3 aliquots. The pooled samples were prepared identically to the individual study samples. 1H NMR spectra of urine samples were acquired on a Bruker Avance 950 MHz NMR spectrometer (located at the David H. Murdock Research Institute at Kannapolis, NC, USA) using a 3 mm cryogenically cooled ATMA inverse probe and ambient temperature of 25℃. A 1D NOESY presaturation pulse sequence (noesypr1d, [recycle delay (RD)-90°-t1-90°-tm-90°-acquire free induction decay (FID) was used for data acquisition. For each sample 64 transients were collected into 65k data points using a spectral width of 14.01 kHz (20.14 ppm), 2 s relaxation delay, 100 ms mixing time, and an acquisition time of 2.324 s per FID. The water resonance was suppressed using resonance irradiation during the relaxation delay and mixing time. NMR spectra were processed using Chenomx NMR Suite 7.51 Professional (Chenomx, Edmonton, Alberta, Canada) software. Spectra were zero filled, and Fourier transformed after exponential multiplication with line broadening factor of 0.5. Phase and baseline of the spectra were manually corrected for each spectrum. Spectra were referenced internally to the DSS signal. The quality of each NMR spectrum was assessed for the level of noise and alignment of identified markers. Spectra were assessed for missing data and underwent quality checks. NMR bins (0.50-9.0 ppm) were made after excluding DSS, water (4.68-4.80 ppm), and Imidazole (7.20-7.28 ppm) using bucket Integration with a 0.04 ppm bucket width. Integrals of each of the bins were normalized to total integral of each of the spectrum. |
Sampleprep Protocol Filename: | RTI_NeonatalAKI_Metabolomics_Procedure.docx |