Summary of study ST000102

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000094. The data can be accessed directly via it's Project DOI: 10.21228/M8X309 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000102
Study TitleCold adaptation shapes the robustness of metabolic networks in Drosophila melanogaster
Study TypeTime course during cold exposure for four genetic lines of flies
Study SummaryMetabolites of cold hardy versus cold susceptible flies were compared using N less than R-based metabolomics. We used 8 replicates per line (2 hardy lines, two susceptible lines), and sampled each line at three time points (before, during and after cold), giving rise to 96 samples total.
Institute
University of Florida
DepartmentEntomology and Nematology
Last NameWilliams
First NameCaroline
Emailcmw@berkeley.edu
Submit Date2014-09-26
Num Groups4
Total Subjects96
Raw Data AvailableYes
Raw Data File Type(s).txt,out, .xml, .par, pdata directory has binary files
Uploaded File Size151 M
Analysis Type DetailNMR
Release Date2015-02-03
Release Version1
Caroline Williams Caroline Williams
https://dx.doi.org/10.21228/M8X309
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000117
Sampleprep Protocol Filename:sample_prep_protocol_24Sept2014.docx
sample_spiking_protocol_24Sept2014.docx
Sampleprep Protocol Comments:Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-7.
Processing Method:lyophilization, homogenization, solvent extraction
Processing Storage Conditions:store at -20C
Extraction Method:modified Bligh and Dyer protocol
Extract Concentration Dilution:2:2:1.8 (methanol:chloroform:water)
Extract Storage:-20C
Sample Resuspension:Samples were re-suspended in 620 ?l buffer (100 mM potassium phosphate [pH 7.3], 0.1% sodium azide, 1 mM trimethylsilylproprionate [TMSP] in D2O)
Sample Spiking:6 internal standards (proline, ?-alanine, tyrosine, histidine, phosphocholine, and 3-hydroxykynurenine) added: 6 ?L of standards were added to a concentration of 2x the Chenomx-determined concentration for each metabolite
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