Summary of Study ST000405

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000317. The data can be accessed directly via it's Project DOI: 10.21228/M87G7J This work is supported by NIH grant, U2C- DK119886.


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Study IDST000405
Study TitleMetabolomic Profiling in Early Pregnancy using Pre-Diagnostic Sera from Women Who Developed Placental Abruption
Study TypeMetabolomics Biocrates Panel
Study SummaryPlacental abruption (PA) is an ischemic placental disorder that results from the premature separation of the placenta from the wall of the uterus before delivery of the fetus. This disorder is associated with pre-term delivery, fetal death, maternal hemorrhagic shock, and renal failure. Several physiologic disturbances, such as oxidative stress, carbohydrate/fatty acid metabolism, and mitochondrial dysfunction, have been associated with ischemic placental disorder as well as with placental abruption. This preliminary study proposes to identify metabolites associated with incident placental abruption using existing serum and clinical data from a previously studied cohort. Metabolomics analysis will be carried out on maternal serum (51 cases and 51 controls) collected in early pregnancy (early second trimester).
RTI International
DepartmentDiscovery Sciences
LaboratorySystems and Translational Sciences
Last NameBurgess
First NameJason
Address3040 Cornwallis Rd, RTP, NC, 27609, USA
Submit Date2016-05-27
Num Groups4
Total Subjects102
Num Females102
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-05-27
Release Version1
Jason Burgess Jason Burgess application/zip

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Sample Preparation:

Sampleprep ID:SP000433
Sampleprep Summary:Sample Preparation Prior to Biocrates p180 Kit Plate Analysis: Serum samples were thawed on ice and pooled QC samples were prepared. Samples (study and pooled QC samples) were then diluted, vortexed, and transferred into properly labeled 2 mL Lo-Bind eppendorf tubes and dried on a lyophilizer overnight. The residue was reconstituted with 30 µL of PBS buffer. Samples were then vortexed and then centrifuged at room temperature and at 16,000 rcf for 4.0 minutes. All study samples and pooled QC samples were splitted into two AbsoluteIDQ p180 Kit-96 wells plates (Plate 1502 and 1503). Biocrates Plate Preparation: The Biocrates p180 kit-96 wells plates were prepared following the AbsoluteIDQ p180 Kit metabolomics procedure. Briefly, an internal standard mix was added to 95 of the 96 wells. Next, zero samples, QC standards and calibration standards were added to their corresponding wells. The study samples and pooled QC Samples were then added to the appropriate wells and dried for 30 minutes under nitrogen flow. The plate was derivatized using a 5% Phenylisothiocyanate (PITC) solution in (1:1:1) Ethanol:Pyridine:Water and, then, incubated for 20 minutes followed by a drying step under nitrogen flow. An extraction solvent (5 mM ammonium acetate in methanol) was added to all wells. The plate was then vortexed and filtrated by centrifugation. After centrifugation, 150 µL was removed and transferred to a second 96-well plate (LC-MS/MS plate). This second plate was diluted with 150 µL of HPLC grade water for a subsequent LC-MS/MS assay (analysis of amino acids and biogenic amines). All the wells in the original plate was diluted with 400 µL of flow injection analysis (FIA) Running Solvent for a FIA-MS analysis (lipids, acylcarnitines, and hexose analysis).