Summary of Study ST000978
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000668. The data can be accessed directly via it's Project DOI: 10.21228/M8B69G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000978 |
Study Title | Metabolomics biomarkers and the risk of overall mortality and ESRD in CKD: results from the Progredir Cohort. |
Study Type | ongoing cohort study |
Study Summary | 454 CKD participants were originally recruited from the outpatient services from Hospital das Clinicas, Sao Paulo. Extensive baseline data and biobank were collected between 2012-2013 in the Clinical Research Center, Universitary Hospital, Sao Paulo. Participants are being actively followed for events of mortality, renal replacement therapy and CVD. Death certificates are obtained from State and National Registries. For the this uploaded data, events were determined up to May 2017 (median follow-up time of 3 y). Censoring data was considered as the last day of contact. |
Institute | University of Sao Paulo |
Department | Clinical Research Center, Universitary Hospital, Sao Paulo University |
Laboratory | Laboratory of Genetics and Molecular Cardiology, Heart Institute |
Last Name | Titan |
First Name | Silvia |
Address | 255 Av Dr Eneas Carvalho Aguiar, Cerqueira César, Sao Paulo, Sp, Brazil, 05403-000 |
smotitan@gmail.com | |
Phone | +1-413-362-4377 |
Submit Date | 2018-05-22 |
Total Subjects | 451 |
Study Comments | Events are overall mortality, RRT, and a composite outcome of mortality and RRT. In addition, we also provided the variable for competitive data analysis (RRT considering the competing effect of death). Please see below for description of Study Design. Cox_mortality: death events (for Cox proportional hazard models) Cox_RRT: renal replacement therapy events (for Cox proportional hazard models) Cox_composite_deathRRT: death events AND renal replacement therapy events (for Cox proportional hazard models) Competitive_RRTvs death: death or renal replacement therapy events (for competitive risk analysis) Time_death: follow-up time for death events and censored-participants (for Cox models) Time_Cox_RRT: follow-up time for renal replacement therapy events and censored-participants (for Cox models) Time_composite_deathRRT: follow-up time for death AND renal replacement therapy and censored-participants (for Cox models) Time_competitive_TRSvsDeath: follow-up time for renal replacement therapy, death or censored-participants (for competitive analysis) |
Analysis Type Detail | GC-MS |
Release Date | 2018-07-17 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001024 |
Sampleprep Summary: | 70uL os serum was thawed on ice and added 300uL of acetronile, isopropanol and water solution (3:3:2 v/v) followed by spike of 5uL of d27 myristic acid (3mg/ml) as internal standard. Samples were vortexed and centrifuged to 15800xg during 15 minutes at 4C. 320uL of supernatante were transferred to a new tube and dried in speedvac during 18 hours. Residues were suspended in 50µL methoxyamine in pyridine solution (40mg/mL), 3µL of FAME (Fatty acid methyl ester ) was added, and the mixture was vortexed for 3 min. This methoximation reaction was performed at room temperature for 16h, followed by trimethylsilylation for 1h adding 100µL MSTFA (N-methyl-N-trimethylsilyltrifluoroacetamine) with 1% TMCS (trimethylchlorosilane) (Sigma-Aldrich). After derivatization, 1µL of this derivative was used for Gas Chromatography Mass Spectrometry (GC/MS) analysis. Storage condition: Desiccator at 4 degree |
Processing Method: | Protein Precipitation followed by metabolite extraction |
Processing Storage Conditions: | On ice |
Extraction Method: | Acetonitrile:Isopropanol: water(3:3:2 v/v) |
Extract Storage: | Described in summary |
Sample Resuspension: | Methoxyamine in pyridine |
Sample Derivatization: | MSTFA + 1% TMCS |
Sample Spiking: | 5uL of d27 Myristic Acid (3mg/mL) and FAME |