Summary of study ST001037

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000694. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT2M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001037
Study TitleHigh Resolution GC-MS and FID Metabolomics of Human Serum
Study TypeHuman Serum Analysis
Study SummaryWe explored the possibility of performing quantitative metabolomics using a QEGC-MS using both FID and MS detectors as complementary techniques.
Institute
Wake Forest Baptist Medical Center
DepartmentCenter for Precision Medicine
LaboratoryMichael Olivier Laboratory
Last NameMisra
First NameBiswapriya
AddressMedical Center Boulevard NRC Building, G#43, Medical Center Boulevard, Winston Salem, NC, 27157, USA
Emailbbmisraccb@gmail.com
Phone3522156040
Submit Date2018-08-13
Total Subjects1
Num Males1
Study CommentsNA
PublicationsIn process
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailGC-MS
Release Date2019-01-22
Release Version1
Biswapriya Misra Biswapriya Misra
https://dx.doi.org/10.21228/M8ZT2M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001083
Sampleprep Summary:Serum samples (30 µL) were subjected to sequential solvent extraction once each with 1 mL of acetonitrile: isopropanol: water (3:3:2, v/v) ratio and 500 µL of acetonitrile: water (1:1, v/v) ratio mixtures at 4 C.22 Adonitol and d4-succinic acid (both 5 µL from 10 mg/ml stock) were added to each aliquots as two internal standards prior to the extraction. The pooled extracts (~ 1500 µL) from the two steps were dried under vacuum at 4 C prior to chemical derivatization. Dummy extractions performed on blank tubes served as extraction blanks to account for background (extraction solvent, derivatization reagents) noise and other sources of contamination (septa, liner, column, vials, handling etc.). Blanks were only used to see that no carry overs occurred during randomized run orders and to manually filter out contaminating chemicals from the combined list of features. Samples were then sequentially derivatized with methoxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) as described elsewhere.23,24 Steps involved addition of 10 μL of MeOX (20 mg/mL) in pyridine incubated under shaking at 55 °C for 60 min followed by trimethylsilylation at 60 °C for 60 min after adding 90 μL MSTFA.
Sampleprep Protocol ID:Fiehn et al., 2008
Sampleprep Protocol Comments:NA
Processing Method:Fiehn et al., 2008
Processing Storage Conditions:On ice
Extraction Method:Fiehn et al., 2008
Extract Enrichment:None
Extract Cleanup:None
Sample Resuspension:NA
Sample Derivatization:Methoxyaminatin + silylation (MSTFA)
Sample Spiking:Adonitol
Subcellular Location:NA
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