Summary of Study ST001041
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000697. The data can be accessed directly via it's Project DOI: 10.21228/M8KH4V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001041 |
| Study Title | Development of a weaned pig model of enterotoxigenic E.coli-induced environmental enteropathy |
| Study Type | Animal Model |
| Study Summary | Environmental Enteropathy (EE) is a subclinical condition primarily effecting developing countries believed to be caused by chronic fecal-oral contamination. The condition is characterized by chronic gut inflammation, malabsorption, stunting of growth, stunted villi, and reduced efficacy of oral vaccines. Due to the similarity of the gastrointestinal tract and immune response of swine and humans, the piglet is an attractive model for studying this condition. In this present study, piglets were challenged with either a chronic or acute dose of pathogenic E.coli in an attempt to mimic the symptoms of EE over a 7 day trial period. Throughout the study a number of biological samples were collected for analysis and are presented here, including feces for untargeted metabolomics analysis. |
| Institute | Texas A&M University |
| Department | Animal Science |
| Laboratory | Gill Lab |
| Last Name | Boeckman |
| First Name | Justin |
| Address | 400 Bizzell Street |
| Jxboeckman@tamu.edu | |
| Phone | 4084893729 |
| Submit Date | 2018-08-21 |
| Num Groups | 3 |
| Total Subjects | 24 |
| Publications | In progress |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001089 |
| Sampleprep Summary: | Massed out 2mg±0.3mg of sample. Add 1ml of 3:3:2 extraction solvent (acetonitrile:isopropanol:water). Add 2 3mm grinder beads. Grind twice for 30 seconds each. Shake for 6 minutes at -4C. Centrifuge for 2 mins at 14000 rcf. Separate two 475µL aliquotes. Centrivape to dry. Add 500 µL ACN:H2O (50:50). Vortex for 10 seconds. Centrifuged for 2 minutes at 14000 rcf. Aliquoted two 475 µL samples. Dry in centrivap. |
