Summary of Study ST001071

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000717. The data can be accessed directly via it's Project DOI: 10.21228/M80M50 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001071
Study TitleGuinea Grass Untargeted Metabolomic Profiling
Study TypeTreatment comparison
Study SummaryTreatment comparison of Guinea grass planted at University of Sao Paulo Brazil 4 Ambient temp and CO2 plots, 4 elevated CO2 (600 ppm) plots, 4 elevated Temp plots (+2C),4 elevated CO2 and Temp plots. Untargeted metabolomics(GC-MS),de novo transcriptomics, and RNAseq taken at 30(A)(2014-05-22) and 50(B)(2014-07-14)days post treatment exposure. Dried leaf material sent to Uni Illinois-UC. GC-MS protocol followed as stated in Ulanov et al. (2010) J of Plant Phys.
University of Illinois Urbana-Champaign
DepartmentPlant Biology
LaboratoryAinsworth USDA ARS Global Change and Photosynthesis Research Unit
Last NameAinsworth
First NameElizabeth
Address1201 W Gregory Dr, Urbana, IL 61801
Submit Date2018-10-09
Num Groups4
Total Subjects32 (+ 4 techincal replicates)
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2018-11-08
Release Version1
Elizabeth Ainsworth Elizabeth Ainsworth application/zip

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Sample Preparation:

Sampleprep ID:SP001122
Sampleprep Summary:Room temp Methanol extration: 10-20 mg leaf material, extracted with 1.5 mL MeOH for 15 min at RT followed by another extraction with 1.4 mL H2O at RT. 1 mL of extraction dried under vacuum. 5 uL internal standard added to each sample. 1uL aliquot per sample
Sampleprep Protocol Comments:As described in Ulanov, A. & J. M. Widholm (2010) Metabolic profiling to determine the cause of the increased triphenyltetrazolium chloride reduction in mannitol-treated maize callus. Journal of Plant Physiology, 167, 1423-1431.
Processing Method:Homogenization and Solvent removal with speed vac
Processing Storage Conditions:Room temperature
Extraction Method:1:1 MeOH/H20
Extract Storage:-80℃
Sample Spiking:Internal Standard (hentriacontanoic acid 5 uL)