Summary of Study ST001071
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000717. The data can be accessed directly via it's Project DOI: 10.21228/M80M50 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001071 |
| Study Title | Guinea Grass Untargeted Metabolomic Profiling |
| Study Type | Treatment comparison |
| Study Summary | Treatment comparison of Guinea grass planted at University of Sao Paulo Brazil 4 Ambient temp and CO2 plots, 4 elevated CO2 (600 ppm) plots, 4 elevated Temp plots (+2C),4 elevated CO2 and Temp plots. Untargeted metabolomics(GC-MS),de novo transcriptomics, and RNAseq taken at 30(A)(2014-05-22) and 50(B)(2014-07-14)days post treatment exposure. Dried leaf material sent to Uni Illinois-UC. GC-MS protocol followed as stated in Ulanov et al. (2010) J of Plant Phys. |
| Institute | University of Illinois Urbana-Champaign |
| Department | Plant Biology |
| Laboratory | Ainsworth USDA ARS Global Change and Photosynthesis Research Unit |
| Last Name | Ainsworth |
| First Name | Elizabeth |
| Address | 1201 W Gregory Dr, Urbana, IL 61801 |
| lisa.ainsworth@ars.usda.gov | |
| Phone | 217-265-9887 |
| Submit Date | 2018-10-09 |
| Num Groups | 4 |
| Total Subjects | 32 (+ 4 techincal replicates) |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-11-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001122 |
| Sampleprep Summary: | Room temp Methanol extration: 10-20 mg leaf material, extracted with 1.5 mL MeOH for 15 min at RT followed by another extraction with 1.4 mL H2O at RT. 1 mL of extraction dried under vacuum. 5 uL internal standard added to each sample. 1uL aliquot per sample |
| Sampleprep Protocol Comments: | As described in Ulanov, A. & J. M. Widholm (2010) Metabolic profiling to determine the cause of the increased triphenyltetrazolium chloride reduction in mannitol-treated maize callus. Journal of Plant Physiology, 167, 1423-1431. |
| Processing Method: | Homogenization and Solvent removal with speed vac |
| Processing Storage Conditions: | Room temperature |
| Extraction Method: | 1:1 MeOH/H20 |
| Extract Storage: | -80℃ |
| Sample Spiking: | Internal Standard (hentriacontanoic acid 5 uL) |
