Summary of Study ST001112

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000743. The data can be accessed directly via it's Project DOI: 10.21228/M8N68W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001112
Study Title	The nutrition value of fish fillet is related to fish maturation or fish age?
Study Summary	Integrated analysis of transcriptomics and metabolomics in Blunt Snout Bream. With the improvement of living standards, people’s demand for food nutrient is getting higher and higher. Fish is one kind of protein-rich food and is increasingly favored by consumers. It has been well recognized that flesh composition of fish is closely related to its maturation and growth stages, but few researches have explored these differences. Besides, hormone residues in fish after artificial inducing reproduction also attract consumers’ concern. In this study, we try to address these concerns by using a combination of transcriptomics and metabolomics analysis to identify the key pathways, genes, and metabolites regulation which may affect flesh nutrition of one typical aquaculture species in China, blunt snout bream (Megalobrama amblycephala).
Institute	Huazhong Agricultural University
Last Name	Guan
First Name	Ningnan
Address	College of Fisheries,Huazhong Agricultural University 1 Shizishan Road Hongshan District Wuhan, Hubei
Email	ningnan06@163.com
Phone	+8613007100773
Submit Date	2017-09-19
Raw Data Available	Yes
Raw Data File Type(s)	bcd
Analysis Type Detail	LC-MS
Release Date	2019-01-22
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001164
Sampleprep Summary:	Muscle tissues about 25 mg from each individual were homogenized in 800 µL cold methanol and water (v/v = 1:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). 400 µL supernatant was collected after centrifugation (25000 g, 4 °C, 10 min). The supernatant in the eppendorf tube was removed and the sediment in the tube was dried. Homogenized the sediment again in 800 µL cold dichloromethane and methanol (v/v = 3:1), after centrifuging and then collected 200 µL supernatant for QC (quality control) sample (25000 g, 4 °C, 10 min). The supernatant was evacuated under vacuum, re-collected 100µL supernatant after centrifugation (25000 g, 4 °C, 10 min) for subsequent detection.

Sampleprep ID:

SP001164

Sampleprep Summary:

Muscle tissues about 25 mg from each individual were homogenized in 800 µL cold methanol and water (v/v = 1:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). 400 µL supernatant was collected after centrifugation (25000 g, 4 °C, 10 min). The supernatant in the eppendorf tube was removed and the sediment in the tube was dried. Homogenized the sediment again in 800 µL cold dichloromethane and methanol (v/v = 3:1), after centrifuging and then collected 200 µL supernatant for QC (quality control) sample (25000 g, 4 °C, 10 min). The supernatant was evacuated under vacuum, re-collected 100µL supernatant after centrifugation (25000 g, 4 °C, 10 min) for subsequent detection.