Summary of Study ST001112

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000743. The data can be accessed directly via it's Project DOI: 10.21228/M8N68W This work is supported by NIH grant, U2C- DK119886.

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Study IDST001112
Study TitleThe nutrition value of fish fillet is related to fish maturation or fish age?
Study Summary Integrated analysis of transcriptomics and metabolomics in Blunt Snout Bream. With the improvement of living standards, people’s demand for food nutrient is getting higher and higher. Fish is one kind of protein-rich food and is increasingly favored by consumers. It has been well recognized that flesh composition of fish is closely related to its maturation and growth stages, but few researches have explored these differences. Besides, hormone residues in fish after artificial inducing reproduction also attract consumers’ concern. In this study, we try to address these concerns by using a combination of transcriptomics and metabolomics analysis to identify the key pathways, genes, and metabolites regulation which may affect flesh nutrition of one typical aquaculture species in China, blunt snout bream (Megalobrama amblycephala).
Institute
Huazhong Agricultural University
Last NameGuan
First NameNingnan
AddressCollege of Fisheries,Huazhong Agricultural University 1 Shizishan Road Hongshan District Wuhan, Hubei
Emailningnan06@163.com
Phone+8613007100773
Submit Date2017-09-19
Raw Data AvailableYes
Raw Data File Type(s)bcd
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Ningnan Guan Ningnan Guan
https://dx.doi.org/10.21228/M8N68W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001171
Treatment Summary:Samples were divided into four groups: 1-year-old immature M. amblycephala females (group Ⅰ), 2-year-old immature M. amblycephala females (group Ⅰ), 2-year-old mature females before the injection of hormones (group Ⅲ) and 2-year-old females after 24 hours of their successfully spawning by injecting oxytocin (group ⅣanThe artificial oxytocin implementation procedure was as follows. Firstly, the fish were intraperitoneally injected with a luteinizing hormone-releasing hormone from the basal part of the pectoral fin (LRH-A2) (1 µg/kg fish body weight). After 10 h, the second injection was given (0.5 ml/kg body weight) (each ml contained LRH-A2 4 µg, human chorionic gonadotropin (HCG) 500 U, and domperidone (DOM) 5 mg of solution).
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