Summary of Study ST001330
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000909. The data can be accessed directly via it's Project DOI: 10.21228/M8668V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001330 |
| Study Title | Multi-omics of OsGF14b-mediated innate immunity against panicle blast in rice |
| Study Summary | In the present study, we used a multi-omics approach to decipher the molecular mechanisms of OsGF14b in governing panicle resistance to Magnaporthe oryzae.Results revealed OsGF14b mediated panicle blast resistance was involved in the activation of auxin and JA signaling pathways, resulting in reprogramming of the phenylpropanoid and diterpenoid pathway. |
| Institute | Agro-biological Gene Research Center , Guangdong Academy of Agricultural Sciences |
| Last Name | Yan |
| First Name | Shijuan |
| Address | No. 20 Jinying Road, Tianhe District, Guangzhou City, Guangdong Province, 510640, China. |
| shijuan@agrogene.ac.cn | |
| Phone | +86-020-38213643 |
| Submit Date | 2020-03-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2020-12-31 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001412 |
| Sampleprep Summary: | The rice panicle pre-cooled in liquid nitrogen were ground using a Mixer/mill (MM400; Retsch) with steel ball for 30 seconds at 30 HZ. Fifty milligram of rice panicle powder of each sample was extracted with a fixed volume (1 ml) of pre-cooled (−20 °C) extraction solvent (methanol: chloroform: water = 5: 2: 2) was added to homogenized tissues. After adding the extraction solvent, the vials/tubes were thoroughly vortexed for 1 min and then incubated on an orbital shaker (200 rpm) for 10 min at 4 °C followed by a 15 min sonication step. For phase separation, a volume of 500 µl of solvent (methanol: water = 1: 3), was added to each vial/tube and the samples were again thoroughly vortexed for 1 min. After that, the samples are centrifuged at a speed of 14000rpm for 10 min at 4 °C. Two fixed volume of 200 μL of the polar phase (the lower phase) were transferred into pre-labeled 1.5 ml microcentrifuge tube respectively. Then the samples were dried in a SpeedVac concentrator without heating. Two dried 200 μL aliquots of the polar phase in each sample were analyzed using gas chromatography tandem mass spectrometry (GC-MS) and liquid chromatography tandem mass spectrometry (LC-MS) for metabolomics study. The dried 200 µl aliquots of the polar phase for GC-MS analysis were re-suspended in methoxyamine-hydrochloride/pyridine solution for methoxymization of carbonyl groups followed by heating at 37 °C for 2 h. The samples were further derivatized with N-methyl-N-trimethylsilyltrifloracetamide (MSTFA) for 30 min at 37 °C. then one µl of the derivatized sample mixture was injected onto the GC-column and measured. Another dried 200 µl aliquots of the polar phase were re-suspended in 150 µl UPLC-grade methanol: water (1:1, vol/vol) and subjected to LC-MS analysis. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
