Summary of Study ST001385
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000949. The data can be accessed directly via it's Project DOI: 10.21228/M8198B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001385 |
| Study Title | Microbial and metabolic variations mediate the influence of childhood and adolescent EDC and trace element exposure on breast density. |
| Study Summary | Our CHEAR project builds on the longitudinal Growth and Obesity Cohort Study in Santiago, Chile, which includes approximately 500 girls born in 2002-2003. Participants were recruited in 2006 from public nursery schools of six counties in Santiago representative of low to middle income families. Children were included if they were singletons with birthweight between 2500 and 4500g, and had no physical, medical or endocrine diseases that may alter the growth and/or onset of puberty. Semi-annual collection of participant anthropometric and pubertal development data by trained dietitians has continued since 2009. The overall objectives of our CHEAR study are to assess how exposure to endocrine-disrupting chemicals (phenols and phthalates), and trace elements (metals) measured in urine samples at Tanner stages 1 and 4 and 1 year post-menarche influence the pubertal microbiome and metabolome, and the potential impact on adolescent breast composition measured by DXA at Tanner stage 4 and 2 years post-menarche. |
| Institute | Icahn School of Medicine at Mount Sinai |
| Last Name | Petrick |
| First Name | Lauren |
| Address | Department of Environmental Medicine and Public Health, Atran Building 3rd floor, 101st St. between Madison and 5th Ave, New York, New York, 10029, USA |
| lauren.petrick@mssm.edu | |
| Phone | 212 241 7351 |
| Submit Date | 2020-05-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Chear Study | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-03-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001467 |
| Sampleprep Summary: | Aliquoting was initially performed for all CHEAR assays, and included an aliquot of each sample for metabolomics and specific gravity measurements. Specific gravity measurements were made first, to determine dilution factors required for pre-acquisition normalization. Specific gravity and dilution factors are included in the “Meta_2019_Michels2017_1977.csv” Urine samples were thawed on ice in batches of approximately 65 samples, and vortexed. The sample was diluted with water down to a specific gravity of 1.002 for pre-acquisition normalization. A 20 L aliquot of the diluted sample was prepared for metabolomics analysis. In addition, a 20 L aliquot from each diluted sample was combined for use as a pooled quality control sample and re-aliquoted into 20L samples. Samples were then returned to -80°C until analysis. Extraction was performed in batches of approximately 65 samples, immediately prior to LC-HRMS analysis. All samples in a batch were thawed on ice, combined with 180L of acetonitrile containing internal standards, and vortexed for 30sec. Samples were then centrifuged (13000 g, 15 min, °C), and 60 L of supernatant transferred to two LC vials for RP and HILIC analysis. Extract remainder was returned to -80°C. Following the same protocol 20 L aliquots each of a matrix blank (replacing the urine with H2O, “matrix”), a CHEAR Reference urine sample (global quality control, “UT”), a NIST 3672 sample (global quality control, “NIST”), and multiple pooledQC samples (local quality control, “LQC”) were extracted. |
| Sampleprep Protocol Filename: | Report_Michels_ 2017_1977.docx |
