Summary of study ST001428

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000980. The data can be accessed directly via it's Project DOI: 10.21228/M8169P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001428
Study TitleRole of environmental toxicants in modulating disease severity in children with NAFLD
Study SummaryThis project aims to further understand about how the environment impacts nonalcoholic fatty liver disease NAFLD and nonalcoholic steatohepatitis (NASH) in children. At present the NASH CRN has the largest, well characterized cohort of children with NAFLD, including clinical data, labs, cytokines, genetic polymorphisms, but no proteomics, metabolomics, lipidomics or toxicant assessment. Exposure and untargeted metabolomics analyses will examine the role of environmental toxicants in modulating disease severity and the endogenous response to exposures in children with NAFLD.
Institute
Emory University
DepartmentSchool of Medicine
LaboratoryClincal Biomarkers Laboratory
Last NameTran
First NameViLinh
Address615 Michael St, suite 225, Atlanta GA 30322
Emailvtran6@emory.edu
Phone9122281788
Submit Date2020-07-17
Total Subjects427
Study CommentsBoth CHEAR pooled plasma samples and Clinical Biomarker Laboratory pooled plasma samples were used
Raw Data AvailableYes
Raw Data File Type(s).raw
Chear Study2017-1593
Analysis Type DetailLC-MS
Release Date2020-07-30
Release Version1
ViLinh Tran ViLinh Tran
https://dx.doi.org/10.21228/M8169P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001510
Sampleprep Summary:Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, plasma aliquots were removed from storage at -80 degrees C and thawed on ice. Each cryotube was then vortexed briefly to ensure homogeneity, and 50 microliters was transferred to a clean microfuge tube. Immediately after, the plasma was treated with 100 microliters of ice-cold LC-MS grade acetonitrile (Sigma Aldrich) containing 2.5 microliters of internal standard solution with eight stable isotopic chemicals selected to cover a range of chemical properties. Following addition of acetonitrile, urine was equilibrated for 30 min on ice, upon which precipitated proteins were removed by centrifuge (14,000 rpm at 4 degrees C for 10 min). The resulting supernatant (100 microliters) was removed, added to a low volume autosampler vial and maintained at 4 degrees C until analysis (<22 h).
Sampleprep Protocol ID:HRM_SP_082016_01
Sampleprep Protocol Filename:EmoryUniversity_HRM_SP_082016_01.pdf
Sampleprep Protocol Comments:Date effective: 30 July 2016
Extraction Method:2:1 acetonitrile: sample followed by vortexing and centrifugation
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