Summary of Study ST001434
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000985. The data can be accessed directly via it's Project DOI: 10.21228/M8CH6R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001434 |
Study Title | Untargeted lipidomics of liver to assess the potential protective role in atherosclerosis progression of A12 antibodies infusion into LDLR-/-mice |
Study Type | LC-MS Untargeted Lipidomics |
Study Summary | In order to assess the therapeutic potential of A12 antibodies in atherosclerosis, untargeted lipidomics of liver samples was performed. LDLR-/-mice were treated with a fully murine version of the A12 antibody (mA12-IgG2b), with the isotype control antibody mB1.8-IgG2b (n=16) or with PBS as controls. |
Institute | Centro Nacional de Investigaciones Cardiovasculares Carlos III |
Last Name | Ferrarini |
First Name | Alessia |
Address | Calle de Melchor Fernández Almagro, 3, 28029 Madrid |
aferrarini@cnic.es | |
Phone | +34 914 53 12 00 |
Submit Date | 2020-07-27 |
Num Groups | 3 |
Total Subjects | 18 |
Publications | DOI. 10.1038/s41586-020-2993-2 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2020-09-25 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001516 |
Sampleprep Summary: | Frozen liver tissues were lysed in 0.5X PBS (sample:solvent 1:10 ratio) containing 1 mg/mL butylhydroxytoluene (BHT) solution in methanol (proportion 200 µL per g of tissue), with FastPrep-24 5G instrument (MP Biomedicals, USA). 25 µL aliquots of homogenate were collected and stored at -80°C until lipidomic extraction. Tissue homogenates were thawed and extracted with methyl-tert-butylether (MTBE) as described in [Matyash, V., et al., Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. Journal of lipid research, 2008. 49(5): p. 1137-46]. 900 µL of organic phase were dried-out in speedvac and resuspended in 50 µL of ACN:H2O (20:80, v:v) just before injection. |