Summary of Study ST001439

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000989. The data can be accessed directly via it's Project DOI: 10.21228/M8VH7G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)
Study IDST001439
Study TitleMetabolites in contents of small intestine in wild type and DAOG181R/G181R mice
Study SummaryTo investigate if DAO regulates metabolites in intestinal lumen, we compared metabolites in contents of small intestine in wild type and DAOG181R/G181R mice. All mice have C57BL/6 background and 8 weeks of age.
Institute
Keio University
DepartmentPharmacology
Last NameSuzuki
First NameMasataka
Address35, Shinanomachi, Shinjuku-ku, Tokyo
Emailmasataka.s@keio.jp
Phone+81-3-5363-3750
Submit Date2019-12-17
Analysis Type DetailMS
Release Date2020-08-04
Release Version1
Masataka Suzuki Masataka Suzuki
https://dx.doi.org/10.21228/M8VH7G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Sample Preparation:

Sampleprep ID:SP001521
Sampleprep Summary:For extracting ionic metabolites. approximately 20 mg of contents of small intestine were dissolved in H2O containing internal standards (H3304-1002, HMT, Japan) with ratio of 1:9. After centrifugation, the supernatant was the centrifugically filtered through a Milipore 5000-Da cutoff filter to remove macromolecules for subsequent analysis with CE-TOFMS. For extracting non-ionic metabolites, approximately 20 mg of contents of small intestine were dissolved in 1 mL of methanol containing internal standard (H3304-1002, HMT). After centrifugation, 1o uL of the supernatant was transferred into grass vials for evaporation under nitrogen gas and reconstituted with 200 uL of 50% isopropanol for subsequent analysis with LC-TOFMS.
  logo