Summary of Study ST001439

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000989. The data can be accessed directly via it's Project DOI: 10.21228/M8VH7G This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001439
Study Title	Metabolites in contents of small intestine in wild type and DAOG181R/G181R mice
Study Summary	To investigate if DAO regulates metabolites in intestinal lumen, we compared metabolites in contents of small intestine in wild type and DAOG181R/G181R mice. All mice have C57BL/6 background and 8 weeks of age.
Institute	Keio University
Department	Pharmacology
Last Name	Suzuki
First Name	Masataka
Address	35, Shinanomachi, Shinjuku-ku, Tokyo
Email	masataka.s@keio.jp
Phone	+81-3-5363-3750
Submit Date	2019-12-17
Analysis Type Detail	MS(Dir. Inf.)
Release Date	2020-08-04
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001521
Sampleprep Summary:	For extracting ionic metabolites. approximately 20 mg of contents of small intestine were dissolved in H2O containing internal standards (H3304-1002, HMT, Japan) with ratio of 1:9. After centrifugation, the supernatant was the centrifugically filtered through a Milipore 5000-Da cutoff filter to remove macromolecules for subsequent analysis with CE-TOFMS. For extracting non-ionic metabolites, approximately 20 mg of contents of small intestine were dissolved in 1 mL of methanol containing internal standard (H3304-1002, HMT). After centrifugation, 1o uL of the supernatant was transferred into grass vials for evaporation under nitrogen gas and reconstituted with 200 uL of 50% isopropanol for subsequent analysis with LC-TOFMS.

Sampleprep ID:

SP001521

Sampleprep Summary:

For extracting ionic metabolites. approximately 20 mg of contents of small intestine were dissolved in H2O containing internal standards (H3304-1002, HMT, Japan) with ratio of 1:9. After centrifugation, the supernatant was the centrifugically filtered through a Milipore 5000-Da cutoff filter to remove macromolecules for subsequent analysis with CE-TOFMS. For extracting non-ionic metabolites, approximately 20 mg of contents of small intestine were dissolved in 1 mL of methanol containing internal standard (H3304-1002, HMT). After centrifugation, 1o uL of the supernatant was transferred into grass vials for evaporation under nitrogen gas and reconstituted with 200 uL of 50% isopropanol for subsequent analysis with LC-TOFMS.