Summary of Study ST001611

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001036. The data can be accessed directly via it's Project DOI: 10.21228/M8SD7T This work is supported by NIH grant, U2C- DK119886.


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Study IDST001611
Study TitleMouse model of sarcoma (STS) to characterize tumor vulnerabilities and identify novel targets for anti-cancer treatment
Study SummaryFor metabolomics study, tumor-bearing mice were starved for 6 hours before sarcoma and skeletal muscles were harvested.
North Carolina State University
Last NameLiu
First NameXiaojing
AddressPolk Hall, RM 128
Submit Date2020-11-10
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-05-11
Release Version1
Xiaojing Liu Xiaojing Liu application/zip

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Sample Preparation:

Sampleprep ID:SP001694
Sampleprep Summary:The tumor sample was first homogenized in liquid nitrogen and then 5 to 10 mg was weighed in a new Eppendorf tube. Ice cold extraction solvent (250 ul 80% MeOH/water) was added to the tissue sample, and a pellet mixer was used to further break down the tissue chunk and form an even suspension, followed by the addition of 250 ul to rinse the pellet mixer. After incubation on ice for an additional 10 min, the tissue extract was centrifuged with a speed of 20,000 g at 4 °C for 10 min. The dry pellets were reconstituted into 30 ul (per 3 mg tissue) sample solvent (water:methanol: acetonitrile, 2:1:1, v/v), and 3 ul was injected to LC-HRMS. 5 ul mouse plasma was mixed with 5 ul water, and 40 ul ice cold methanol was added. After vortex for 1 min, the mixture was centrifuged with a speed of 20,000 g at 4 °C for 10 min, and 3 l was injected to LC-HRMS.