Summary of Study ST001650

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001056. The data can be accessed directly via it's Project DOI: 10.21228/M86D8X This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001650
Study TitleChanges to gut microbiota metabolism based on Clostridium difficile-induced inflammation
Study SummaryDetermine changes to metabolites in lumen (cecal contents) of mice due to toxin production by Clostridium difficile R20291.
Stanford University
DepartmentMicrobiology & Immunology
Last NameUniversity
First NameStanford
Address299 Campus Drive Fairchild Building Rm D315
Submit Date2020-12-21
Num Groups3
Total Subjects14
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2021-01-25
Release Version1
Stanford University Stanford University application/zip

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Sample Preparation:

Sampleprep ID:SP001733
Sampleprep Summary:Metabolites were extracted according to the methodology described in Fiehn 2017 Curr Protoc Mol Biol. ; 114: 30.4.1–30.4.32. doi:10.1002/0471142727.mb3004s114. Briefly, 1 mL 3:3:2 acetonitrile:isopropanol:water was added to ~4 mg cecal contents/feces. Samples were homogenized and then shaken at 4 C for 6 min prior to centrifugation at 14,000 x g for 2 minutes. An aliquot of 475 µl supernatant was taken for subsequent analysis, evaporated, cleaned with acetonitrile and water (1:1), and fully evaporated to dryness. 10 µl MeOH and 91 µl MSTFA were added to all samples for derivatization.