Summary of Study ST001749

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001121. The data can be accessed directly via it's Project DOI: 10.21228/M8SX23 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001749
Study TitleREACH Metabolomics Study
Study SummaryIncreasing evidence indicates that physical activity and exercise training may delay or prevent the onset of Alzheimer’s disease (AD). However, systemic biomarkers that can measure exercise effects on brain function and that link to relevant metabolic responses are lacking. This study utilized blood samples of 23 asymptomatic late middle-aged adults with familial and genetic risk for AD who underwent 26 weeks of supervised treadmill training. Metabolomic profiles were evaluated using MS.
University of Wisconsin - Madison
LaboratoryWisconsin Alzheimer's Disease Research Center
Last NameGaitán
First NameJulian
Address600 Highland Ave. J5/1M CSC MC2420, Madison, WI 53792
Submit Date2021-04-15
Num Groups2
Total Subjects23
Num Males12
Num Females11
Analysis Type DetailLC-MS
Release Date2021-05-11
Release Version1
Julian Gaitán Julian Gaitán application/zip

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Sample Preparation:

Sampleprep ID:SP001832
Sampleprep Summary:Samples were prepared using the MicroLab STAR system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.