Summary of Study ST001749

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001121. The data can be accessed directly via it's Project DOI: 10.21228/M8SX23 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001749
Study Title	REACH Metabolomics Study
Study Summary	Increasing evidence indicates that physical activity and exercise training may delay or prevent the onset of Alzheimer’s disease (AD). However, systemic biomarkers that can measure exercise effects on brain function and that link to relevant metabolic responses are lacking. This study utilized blood samples of 23 asymptomatic late middle-aged adults with familial and genetic risk for AD who underwent 26 weeks of supervised treadmill training. Metabolomic profiles were evaluated using MS.
Institute	University of Wisconsin - Madison
Department	Medicine
Laboratory	Wisconsin Alzheimer's Disease Research Center
Last Name	Gaitán
First Name	Julian
Address	600 Highland Ave. J5/1M CSC MC2420, Madison, WI 53792
Email	jgaitan@medicine.wisc.edu
Phone	608-263-2245
Submit Date	2021-04-15
Num Groups	2
Total Subjects	23
Num Males	12
Num Females	11
Analysis Type Detail	LC-MS
Release Date	2021-05-11
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001832
Sampleprep Summary:	Samples were prepared using the MicroLab STAR system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.

Sampleprep ID:

SP001832

Sampleprep Summary:

Samples were prepared using the MicroLab STAR system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.