Summary of Study ST001905

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001200. The data can be accessed directly via it's Project DOI: 10.21228/M8KX3M This work is supported by NIH grant, U2C- DK119886.


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Study IDST001905
Study TitleMetabolomic profiling of saliva in diabetes patients
Study SummaryWe performed comprehensive profiling of plasma and salivary metabolomes, and investigated multivariate covariations with clinical markers of oral and cardiometabolic health in healthy subjects and type 2 diabetes patients. The key findings highlight the potential utility of salivary metabolites for reflecting cardiometabolic changes, including hyperglycemia and dyslipidemia. Our study shows that analysis of panels of salivary metabolites may become an attractive alternative to blood testing for screening of metabolic disorders.
Osaka University
Last NameSakanaka
First NameAkito
Address1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
Submit Date2021-08-15
Analysis Type DetailGC-MS
Release Date2022-12-09
Release Version1
Akito Sakanaka Akito Sakanaka application/zip

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Sample Preparation:

Sampleprep ID:SP001989
Sampleprep Summary:Saliva samples were thawed at 4℃, then vortexed and centrifuged (4 ℃, 18,000 × g) for 3 min. Next, 0.8 mL of the aqueous layer was pipetted off and weighed, then 0.3 mL of that was transferred into a 2-mL glass vial (Nichiden-Rika Glass, Kobe, Japan) and kept at 4℃ in a CubeCooler®. For extraction, 0.3 mL of deaerated MilliQ water containing ribitol (0.02 mg/mL) as an internal standard was added. After incubation using an Eppendorf thermomixer (25℃, 1000 rpm, 10 min), 1.4 mL of deaerated acetonitrile was added. After incubation (25℃, 1000 rpm, 10 min) and centrifugation (4℃, 1800 × g) for 3 min, 1.6 mL of the supernatant was transferred to a 2-mL tube and dried with a vacuum concentrator (VC-96R; TAITEC, Koshigaya, Japan) for 30 min, then allowed to lyophilize overnight. Derivatization was performed with a methoxyamine hydrochloride solution with pyridine at a concentration of 20 mg/mL, followed by silylation application of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA).