Summary of Study ST001946

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001233. The data can be accessed directly via it's Project DOI: 10.21228/M8B99H This work is supported by NIH grant, U2C- DK119886.

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Study IDST001946
Study TitleBipolar disorder metabolomics analysis using FiehnLib and GMD for curation
Study TypeUntargeted GC analysis
Study SummaryIn this study we analyzed the blood serum from 14 controls and 14 BD patients using GC-MS under the conditions required for the use of FiehnLib library with GMD as secondary library for curation on a untargeted metabolomics approach
Institute
University of Campinas
DepartmentChemistry Institute, Department of Analytical Chemistry
LaboratoryLaboratory of Bioanalytics and Integrated Omics (LaBIOmics)
Last NameRibeiro
First NameHenrique
AddressRua Sérgio Buarque de Holanda,s/n
Emailhcarachoribeiro@gmail.com
Phone+55 19 3521 3018
Submit Date2021-10-11
Num Groups2
Total Subjects28
Num Males11
Num Females17
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2021-11-04
Release Version1
Henrique Ribeiro Henrique Ribeiro
https://dx.doi.org/10.21228/M8B99H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002030
Sampleprep Summary:The metabolites from serum samples were extracted by a simple protein precipitation method, using 50 µL of serum and 150 µL of MeOH and 150 µL of pentadecanoic acid as the internal standard. The mixture was vortexed, centrifuged at 15000 RPM, subsequently separating and discarding the protein phase. The aqueous phase was then dried in a SpeedVac at room temp and the dried samples were then submitted to a two-step derivatization, where 10 µL of methoxamine hydrochloride pyridine solution (40 mg mL-1) was added to the sample, kept at 30 ºC for 90 minutes. After the first derivatization step, 90 µL of MSTFA with 1 % (v/v) of TMCS (trimethylchlorosilane) was added, with the sample being kept at 37 ºC for 30 minutes.
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