Summary of Study ST001946

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001233. The data can be accessed directly via it's Project DOI: 10.21228/M8B99H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001946
Study Title	Bipolar disorder metabolomics analysis using FiehnLib and GMD for curation
Study Type	Untargeted GC analysis
Study Summary	In this study we analyzed the blood serum from 14 controls and 14 BD patients using GC-MS under the conditions required for the use of FiehnLib library with GMD as secondary library for curation on a untargeted metabolomics approach
Institute	University of Campinas
Department	Chemistry Institute, Department of Analytical Chemistry
Laboratory	Laboratory of Bioanalytics and Integrated Omics (LaBIOmics)
Last Name	Ribeiro
First Name	Henrique
Address	Rua Sérgio Buarque de Holanda,s/n
Email	hcarachoribeiro@gmail.com
Phone	+55 19 3521 3018
Submit Date	2021-10-11
Num Groups	2
Total Subjects	28
Num Males	11
Num Females	17
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	GC-MS
Release Date	2021-11-04
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002030
Sampleprep Summary:	The metabolites from serum samples were extracted by a simple protein precipitation method, using 50 µL of serum and 150 µL of MeOH and 150 µL of pentadecanoic acid as the internal standard. The mixture was vortexed, centrifuged at 15000 RPM, subsequently separating and discarding the protein phase. The aqueous phase was then dried in a SpeedVac at room temp and the dried samples were then submitted to a two-step derivatization, where 10 µL of methoxamine hydrochloride pyridine solution (40 mg mL-1) was added to the sample, kept at 30 ºC for 90 minutes. After the first derivatization step, 90 µL of MSTFA with 1 % (v/v) of TMCS (trimethylchlorosilane) was added, with the sample being kept at 37 ºC for 30 minutes.

Sampleprep ID:

SP002030

Sampleprep Summary:

The metabolites from serum samples were extracted by a simple protein precipitation method, using 50 µL of serum and 150 µL of MeOH and 150 µL of pentadecanoic acid as the internal standard. The mixture was vortexed, centrifuged at 15000 RPM, subsequently separating and discarding the protein phase. The aqueous phase was then dried in a SpeedVac at room temp and the dried samples were then submitted to a two-step derivatization, where 10 µL of methoxamine hydrochloride pyridine solution (40 mg mL-1) was added to the sample, kept at 30 ºC for 90 minutes. After the first derivatization step, 90 µL of MSTFA with 1 % (v/v) of TMCS (trimethylchlorosilane) was added, with the sample being kept at 37 ºC for 30 minutes.