Summary of Study ST001981

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001257. The data can be accessed directly via it's Project DOI: 10.21228/M87986 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001981
Study Title	Non-destructive characterization of Mesenchymal stem cells
Study Summary	Culture media from the growth of three different MSC cell lines (two bone marrow, one iPSC) were sampled daily for NMR metabolomics analysis. T cell proliferation and IDO assays were used as surrogates of anti-inflammatory function. Linear regression was used to assess the media metabolic changes over time, and partial least squares regression (PLSR) was then used to obtain predictive media markers (PMMs) based on variable importance in projection (VIP) scores. In addition, pathway analysis was performed to show the relations between media metabolites (MMs) and cell metabolites (CMs).
Institute	University of Georgia
Last Name	Shen
First Name	Xunan
Address	315 riverbend road
Email	xs41379@uga.edu
Phone	7858407009
Submit Date	2021-09-16
Raw Data Available	Yes
Analysis Type Detail	NMR
Release Date	2021-12-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002068
Sampleprep Summary:	One hundred µl culture media was thawed on ice and then centrifuged at 14,000 x g for 15 min at 4◦C. For each sample, 54 µl of media supernatant was transferred to a new Eppendorf tube. 30 µl of remaining media in each sample from the same cell line was pooled together to generate six 54 µl internal quality control (QC) samples. 6 µl of 10/3 mM DSS-D6 (Cambridge Isotope Laboratory) in D2O (Cambridge Isotope Laboratory) was then added into each sample. In addition, six buffer blanks (6 µl of 10/3 mM DSS-D6 in 54 µl D2O) samples were added in each cell line for quality assurance purpose. We had 10 replicates for each cell line and time point. A total of 262 samples including 250 experimental samples, 6 pooled samples and 6 buffer blanks were generated for the iMSC group. A total of 222 samples including 210 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM71. A total of 242 samples including 230 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM182. All samples were randomized to reduce technical variance.

Sampleprep ID:

SP002068

Sampleprep Summary:

One hundred µl culture media was thawed on ice and then centrifuged at 14,000 x g for 15 min at 4◦C. For each sample, 54 µl of media supernatant was transferred to a new Eppendorf tube. 30 µl of remaining media in each sample from the same cell line was pooled together to generate six 54 µl internal quality control (QC) samples. 6 µl of 10/3 mM DSS-D6 (Cambridge Isotope Laboratory) in D2O (Cambridge Isotope Laboratory) was then added into each sample. In addition, six buffer blanks (6 µl of 10/3 mM DSS-D6 in 54 µl D2O) samples were added in each cell line for quality assurance purpose. We had 10 replicates for each cell line and time point. A total of 262 samples including 250 experimental samples, 6 pooled samples and 6 buffer blanks were generated for the iMSC group. A total of 222 samples including 210 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM71. A total of 242 samples including 230 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM182. All samples were randomized to reduce technical variance.