Summary of Study ST001990
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001264. The data can be accessed directly via it's Project DOI: 10.21228/M8B39F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001990 |
| Study Title | Metabolomics of the interaction between a consortium of entomopathogenic fungi and their target insect: mechanisms of attack and survival |
| Study Type | Untargeted Metabolomics |
| Study Summary | One of the most concerning pests that attack strawberries in Brazil is Duponchelia fovealis, a non-native moth with no registered control methods to date. Our group recently observed that a fungal consortium formed by two strains of Beauveria bassiana increased the mortality of D. fovealis more than inoculation with each strain on its own. However, the molecular interaction between the fungal consortium and the caterpillars is unknown, raising several questions about the enhanced pest control observed. Furthermore, concerns over the emergency of resistance and the selection for resistance to chemical and biological products that are constantly applied in agriculture highlight the need for careful examination of novel pest control methods. Thus, in this work, we sought to pioneer the evaluation of the molecular interaction between a fungal consortium of B. bassiana and D. fovealis caterpillars. We aimed to understand the biocontrol process involved in this interaction and the defense system of the caterpillar. Therefore, seven days after D. fovealis caterpillars were inoculated with the B. bassiana consortium, the dead and surviving caterpillars were analyzed using GC-MS and LC-MS/MS. |
| Institute | Universidade Federal do Paraná |
| Department | Patologia Básica |
| Laboratory | Laboratório de Microbiologia e Biologia Molecular |
| Last Name | Katiski da Costa Stuart |
| First Name | Andressa |
| Address | Av. Cel. Francisco Heráclito dos Santos, 100, Curitiba, Paraná, 81530-000, Brazil |
| andressa.katiski@gmail.com | |
| Phone | 55 41 991922779 |
| Submit Date | 2021-11-12 |
| Num Groups | 7 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, raw(Waters) |
| Analysis Type Detail | API-MS |
| Release Date | 2023-05-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002077 |
| Sampleprep Summary: | After the direct contact bioassay, the caterpillars were arranged by treatment, then macerated in liquid nitrogen (N2). Extraction was performed with 200 mg of the macerate added to a 1 mL microtube (Eppendorf, Germany) previously treated with methanol. Following this, 125 μL of chloroform (CHCl3), 50 μL ultra-pure water (H2O), and 250 μL cold methanol (CH3OH) were added to the macerate. The microtubes were vigorously vortexed and placed in an ultrasonic bath (Odontobrás, Ribeirão-SP) at 20 Hz and approximately 4ºC for 10 minutes. Then, 50 μL of CHCl3 and 50 μL of H2O were added, and the tubes were vortexed again. The samples were centrifuged (Eppendorf, Germany) for 5 minutes at 14000 rpm and 4°C, and the supernatant was filtered on a Whatman® 0.22 µm filter (Merck, Germany) and transferred to a glass vial. The vial was taken to a lyophilizer (Thermo Fischer Scientific, MA) until the samples had completely dried. Finally, the lyophilized samples were resuspended in 200 µL of extraction solution and aliquoted for use in the GC-MS and LC-MS/MS. |
| Sampleprep Protocol Filename: | Metabolite_Extraction |
| Extract Storage: | -80℃ |
