Summary of Study ST001990

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001264. The data can be accessed directly via it's Project DOI: 10.21228/M8B39F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001990
Study TitleMetabolomics of the interaction between a consortium of entomopathogenic fungi and their target insect: mechanisms of attack and survival
Study TypeUntargeted Metabolomics
Study SummaryOne of the most concerning pests that attack strawberries in Brazil is Duponchelia fovealis, a non-native moth with no registered control methods to date. Our group recently observed that a fungal consortium formed by two strains of Beauveria bassiana increased the mortality of D. fovealis more than inoculation with each strain on its own. However, the molecular interaction between the fungal consortium and the caterpillars is unknown, raising several questions about the enhanced pest control observed. Furthermore, concerns over the emergency of resistance and the selection for resistance to chemical and biological products that are constantly applied in agriculture highlight the need for careful examination of novel pest control methods. Thus, in this work, we sought to pioneer the evaluation of the molecular interaction between a fungal consortium of B. bassiana and D. fovealis caterpillars. We aimed to understand the biocontrol process involved in this interaction and the defense system of the caterpillar. Therefore, seven days after D. fovealis caterpillars were inoculated with the B. bassiana consortium, the dead and surviving caterpillars were analyzed using GC-MS and LC-MS/MS.
Institute
Universidade Federal do Paraná
DepartmentPatologia Básica
LaboratoryLaboratório de Microbiologia e Biologia Molecular
Last NameKatiski da Costa Stuart
First NameAndressa
AddressAv. Cel. Francisco Heráclito dos Santos, 100, Curitiba, Paraná, 81530-000, Brazil
Emailandressa.katiski@gmail.com
Phone55 41 991922779
Submit Date2021-11-12
Num Groups7
Raw Data AvailableYes
Raw Data File Type(s)cdf, raw(Waters)
Analysis Type DetailAPI-MS
Release Date2023-05-12
Release Version1
Andressa Katiski da Costa Stuart Andressa Katiski da Costa Stuart
https://dx.doi.org/10.21228/M8B39F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR002083
Treatment Summary:The treatments applied were: control caterpillars (consisting only of 0.85% saline solution and Tween 80®), caterpillars inoculated with Bov 3 strain of B. bassiana, caterpillars inoculated with Bov 2 strain of B. bassiana and caterpillars inoculated with the Bov3-Bov2 consortium.
Treatment Protocol Filename:Metabolite_Extraction
Treatment Dosevolume:2 x 10^7 conidia/mL
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