Summary of Study ST001993

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001266. The data can be accessed directly via it's Project DOI: 10.21228/M82M5M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001993
Study Title	CE-MS based metabolomics to study plasma samples that reveal new pathways implicated in SARS-CoV-2 pathogenesis
Study Summary	CE-MS based metabolomics was used in this study to analize COVID-19 disease and the susceptibility to SARS-CoV-2. In total, 63 plasma samples were analyzed and different comparisons were performed. To our knowledge, CE-MS has never been used to study COVID-19 and it is considered in this study an appropriate approach to extend the polar metabolome beyond what has been obtained by LC-MS and GC-MS
Institute	Universidad CEU San Pablo
Department	Pharmacy
Laboratory	Centro de Metabolomica y Bioanálisis (CEMBIO)
Last Name	Barbas
First Name	Coral
Address	CEU Universities, Urbanización Montepríncipe
Email	cbarbas@ceu.es
Phone	+34 913724700
Submit Date	2021-11-16
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2021-12-05
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002080
Sampleprep Summary:	Two hundred microliters of frozen supernatant were thawed on ice and evaporated to dryness using a SpeedVac Concentrator System (Thermo Fisher Scientific, Waltham, MA). Then, it was resuspended in 100 µL of 0.2 mM methionine sulfone (MetS) in 0.1 M formic acid. Samples were vortex-mixed for 1 min, transferred to a Millipore filter (30 kDa protein cutoff) and centrifuged for 40 min at 2000 xg at 4 °C. Finally, the ultrafiltrate was transferred to a CE-MS vial for analysis. Quality control samples (QC) were prepared by pooling equal volumes of plasma supernatant from each sample and were treated as previously described. Finally, blank solutions were also prepared with MeOH:EtOH (1:1, v/v).

Sampleprep ID:

SP002080

Sampleprep Summary:

Two hundred microliters of frozen supernatant were thawed on ice and evaporated to dryness using a SpeedVac Concentrator System (Thermo Fisher Scientific, Waltham, MA). Then, it was resuspended in 100 µL of 0.2 mM methionine sulfone (MetS) in 0.1 M formic acid. Samples were vortex-mixed for 1 min, transferred to a Millipore filter (30 kDa protein cutoff) and centrifuged for 40 min at 2000 xg at 4 °C. Finally, the ultrafiltrate was transferred to a CE-MS vial for analysis. Quality control samples (QC) were prepared by pooling equal volumes of plasma supernatant from each sample and were treated as previously described. Finally, blank solutions were also prepared with MeOH:EtOH (1:1, v/v).