Summary of Study ST002262

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001443. The data can be accessed directly via it's Project DOI: 10.21228/M86H66 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002262
Study Title	Metabolomics and metagenomics of pediatric obesity (Feces)
Study Summary	Pediatric obesity has grown as important global health problem in the world. The pediatric obesity affects all the organs and it is closely linked to risks of metabolic diseases such as diabetes, cardiovascular disease, and mental disease. However, the mechanism of both the pediatric obesity and treatment of the obesity remains unclear. Therefore, we investigated metabolomic pathways related to the pediatric obesity and the treatment through metabolomics and metagenomics approaches.
Institute	Seoul National University College of Medicine and Hospital
Department	Department of Clinical Pharmacology and Therapeutics
Last Name	Lee
First Name	Yujin
Address	101 Daehak-ro, Jongno-gu, Seoul 110-799, Korea, Seoul, Seoul, 03080, Korea, South
Email	yoojinlee@snu.ac.kr
Phone	+82-10-3380-4686
Submit Date	2022-07-10
Raw Data Available	Yes
Raw Data File Type(s)	smp
Analysis Type Detail	GC-MS
Release Date	2023-01-04
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002354
Sampleprep Summary:	Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min.

Sampleprep ID:

SP002354

Sampleprep Summary:

Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min.