Summary of Study ST002568

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001655. The data can be accessed directly via it's Project DOI: 10.21228/M8S726 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002568
Study Title	Assessment of metabolic changes in different animal models of maladaptive right ventricular hypertrophy in chronic pulmonary hypertension by lipidomics and HILIC
Study Summary	We analyzed plasma samples from 33 Yucatan pigs belonging to four different experimental models of pulmonary hypertension (PH): M1 chronic postcapillary PH by pulmonary vein banding; M2 chronic PH by aorto-pulmonary shunting; M3: right ventricular pressure overload by pulmonary artery banding, thus without PH; and M0 sham procedure. Briefly, blood samples were collected 8 months after surgery and untargeted lipidomics and HILIC metabolomic analyses were performed for negative and positive polarities. For quality control, 5 QC samples were included in each analysis, iterative MSMS was performed for metabolite annotation. Plasma metabolomic patterns differed among groups, displaying arginine-nitric oxide and histidine deficiency in both PH models (M1 and M2), altered taurine and purine pathways in M2, and lipidomic changes in all three models of pressure overload.
Institute	Universidad CEU San Pablo
Last Name	Moran
First Name	Maria
Address	Universidad CEU-San Pablo. Urb. Montepríncipe
Email	maria.morangarrido@ceu.es
Phone	913724769
Submit Date	2023-03-23
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-05-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002675
Sampleprep Summary:	For the lipidomics protocol 800μl of a solvent mixture with internal standard (made from 75ml chloroform, 75 ml MTBE,100ml MeOH,937.5ul D-palmitic acid and 40 μl C17-sphinganine) were added to 40μl of plasma. Samples were vortexed for 30 seconds, then they were shaken at 1000rpm for 20 min at room temperature, finally they were centrifuged 5 min at 3000 rpm and supernatant was transferred to LC-MS vials. For the HILIC sample preparation 300μl of MeOH were added to 100μl of plasma and it was vortexed for 2 minutes. Samples were left on ice for 10 minutes and then centrifuged at 18,670 g for 10 minutes at 4ºC. Finally, the supernatant was collected and transferred to an LC-MS vial.

Sampleprep ID:

SP002675

Sampleprep Summary:

For the lipidomics protocol 800μl of a solvent mixture with internal standard (made from 75ml chloroform, 75 ml MTBE,100ml MeOH,937.5ul D-palmitic acid and 40 μl C17-sphinganine) were added to 40μl of plasma. Samples were vortexed for 30 seconds, then they were shaken at 1000rpm for 20 min at room temperature, finally they were centrifuged 5 min at 3000 rpm and supernatant was transferred to LC-MS vials. For the HILIC sample preparation 300μl of MeOH were added to 100μl of plasma and it was vortexed for 2 minutes. Samples were left on ice for 10 minutes and then centrifuged at 18,670 g for 10 minutes at 4ºC. Finally, the supernatant was collected and transferred to an LC-MS vial.