Summary of Study ST002765

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001723. The data can be accessed directly via it's Project DOI: 10.21228/M80F0C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002765
Study Title	Disorder of Lipids Induced by Gestational Asthma and its Effect on the Development of Fetal Lung Function and Fetal Health
Study Summary	Maternal asthma during pregnancy is highly correlated with fetal growth and development, and can cause damage to both the mother and fetus, but the underlying mechanisms are not yet clear. Amniotic fluid, as the environment for fetal growth and development, may be affected by lipid metabolism disorders, which can impact fetal lung function development. A rat model of asthma during pregnancy induced by common allergen house dust mite (HDM) was used to investigate changes in lipid composition in amniotic fluid and bronchoalveolar lavage fluid (BALF) by ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS), revealing the impact of maternal asthma during pregnancy on fetal lipid metabolism. In this study, maternal asthma aggravated inflammatory indicators and pathological manifestations after fetal allergen exposure, creating a high oxidative stress growth environment for the fetus, and causing metabolic differences in various lipid groups, including phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and fatty acids (FA), indicating significant lipid metabolism disorders. Improving lipid metabolism may help asthmatic pregnant women maintain healthy fetal development.
Institute	Nanjing University of Chinese Medicine
Last Name	Fang
First Name	Huafeng
Address	No.138 xianlin road, nanjing city, Nanjing, China, 210046, China
Email	Riorofhf@outlook.com
Phone	+86 18852416998
Submit Date	2023-06-29
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-01-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002878
Sampleprep Summary:	500 μL of amniotic fluid was lyophilized and redissolved in 200 μL of deionized water. Then, 80 μL of redissolved amniotic fluid was pipetted off into a 1.5 mL centrifuge tube containing 225 μL of ice-cooled methanol (Merck, Germany) pre-mixed with lyso PE (17:1; LM171LPE-11), SM (17:0; 170SM-13), and PE (17:0/17:0; LM170PE-19) internal standards (5 μg·mL-1; Avanti Polar Lipids, USA). The solution was vortexed for 10 s and added with 750 μL of ice-cooled MTBE. The mixture was shaken for 10 min at 4 °C, added with 188 μL of deionized water, vortexed for 20 s, and centrifuged at 18000 rpm for 2 min at 4 °C. 350 μL of the upper layer (the organic phase, mainly including lipids) and 110 μL of the bottom layer (the aqueous phase, mainly including polar substances) were separately transferred to a new centrifuge tube (1.5 mL). The samples were dried using the Savant SPD1010 vacuum centrifugal concentrator (Thermo Fisher Scientific, USA) and stored at -20 °C before testing. Lipids in the upper layers were lysed with 110 μL of methanol-toluene (9:1) solution.

Sampleprep ID:

SP002878

Sampleprep Summary:

500 μL of amniotic fluid was lyophilized and redissolved in 200 μL of deionized water. Then, 80 μL of redissolved amniotic fluid was pipetted off into a 1.5 mL centrifuge tube containing 225 μL of ice-cooled methanol (Merck, Germany) pre-mixed with lyso PE (17:1; LM171LPE-11), SM (17:0; 170SM-13), and PE (17:0/17:0; LM170PE-19) internal standards (5 μg·mL-1; Avanti Polar Lipids, USA). The solution was vortexed for 10 s and added with 750 μL of ice-cooled MTBE. The mixture was shaken for 10 min at 4 °C, added with 188 μL of deionized water, vortexed for 20 s, and centrifuged at 18000 rpm for 2 min at 4 °C. 350 μL of the upper layer (the organic phase, mainly including lipids) and 110 μL of the bottom layer (the aqueous phase, mainly including polar substances) were separately transferred to a new centrifuge tube (1.5 mL). The samples were dried using the Savant SPD1010 vacuum centrifugal concentrator (Thermo Fisher Scientific, USA) and stored at -20 °C before testing. Lipids in the upper layers were lysed with 110 μL of methanol-toluene (9:1) solution.