Summary of Study ST002918

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001799. The data can be accessed directly via it's Project DOI: 10.21228/M85M79 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002918
Study Title	Metabolic Profiling in mouse Infected with Vibrio parahaemolyticus
Study Summary	Mice were subject to intraperitoneal (i.p.) injection with the V. parahaemolyticus, and control mice were injected with saline. Data were collected and analyzed using unsupervised hierarchical clustering. In general, the abundance of metabolites increased more often than it decreased in animals with higher resistance to infection. Load weight analysis of biological pathways suggested that alanine, aspartate, glutamate metabolism could play roles in the capacity of mice to survive infection with V. parahaemolyticus.
Institute	Sun Yat-sen University
Last Name	jiang
First Name	ming
Address	No. 135, Xingang Xi Road, Guangzhou, 510275, P. R. China
Email	jiangm28@mail.sysu.edu.cn
Phone	13434283781
Submit Date	2023-09-16
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	GC-MS
Release Date	2023-10-05
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003037
Sampleprep Summary:	Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.

Sampleprep ID:

SP003037

Sampleprep Summary:

Spleen tissues were weighed and homogenized with the first solvent (the mixture of chloroform, methanol and water (1:2:1, v/v/v)) for 30 s at 4 0C and then centrifuged at 12,000 rpm for 10 min at 4 0C. The supernatant was collected and deposit was re-homogenized with the second solvent (methanol alone) before a second centrifugation. The two supernatants were mixed, and aliquot of sample was transferred to a GC sampling vial containing 5 μL 0.1 mg/mL ribitol (Sigma) as an analytical internal standard and then dried in a vacuum centrifuge concentrator before the subsequent derivatization. Two technical replicates were prepared for each sample.